Biolegend, 607353, Ultra-LEAF™ Purified anti-human VISTA (PD-1H) Antibody, 100μg

VISTA, V-domain Ig suppressor of T cell activation, suppresses T cell activation and was initially identified by affymetrix analysis of activated versus resting mouse CD25 + CD4 + natural T reg cells. Human Vista is a type I transmembrane protein with a single extracellular IgV domain. It is 311 amino acids (aa) long, contains a 32-aa signal peptide, a 130-aa extracellular IgV domain, 33-aa stalk region, 20-aa transmembrane domain, and 96-aa cytoplasmic tail. VISTA shares significant sequence homology with the B7 family ligands PD-L1 and PD-L2. VISTA is predominantly expressed in hematopoietic tissues, highly expressed in human placenta, which may be indicative of a functional role for VISTA in allofetal tolerance. Flow cytometry shows that human VISTA is expressed in subsets of CD11b hi blood monocytes, dendritic cells, about 20% of CD4, with low density in CD8 T cells, and CD66b + neutrophils at an intermediate intensity. VISTA serves both as a ligand (for antigen presenting cells) and as a receptor (for T cells). B7-H5 expressing on APCs or soluble B7-H5-Ig fusion proteins inhibit T cell proliferation and cytokine production in vitro. B7-H5 also inhibits the expression of T cell activation markers CD44 and CD69. In murine tumor models, anti-VISTA monoclonal antibodies (mAbs) prime to an increased number and elevated function of intratumoral T cells and in that way boost antitumor immunity. VISTA is negative checkpoint regulator and a promising target for cancer immunotherapy.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant human VISTA(PD-1H)
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: Block – Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by blocking the binding of 5 µg/mL biotinylated recombinant human VISTA- Avi to 2 µg/mL immobilized recombinant human IGSF11/BT-IgSF/VSIG3-FC. ND50 range: 1-6 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2927926 (BioLegend Cat. No. 607353) AB_2927926 (BioLegend Cat. No. 607354)
Structure: Dimer
Distribution: Hematopoietic cells, monocytes, macrophages, dendritic cell, granulocytes, subsets of T cells
Function: VISTA suppresses proliferation of T cells and decreases their cytokine production and activation markers.
Interaction: Naïve T cells
Ligand/Receptor: Human recombinant VISTA inhibits the anti CD3-induced proliferation of activated human T cells.
Cell Type: Dendritic cells, Hematopoietic stem and progenitors, Macrophages, Monocytes, T cells
Biology Area: Immunology, Stem Cells
Molecular Family: Soluble Receptors
Antigen References: Wang Li, et al. 2011. J Exp Med. 208:577-92. Zhu Y, et al. 2013. Nat Commun. 4:2043. Ceeraz S, et al. 2013. Trends Immunol. 34:556-63. Le Mercier I, et al. 2014. Cancer Res. 74:1933-44. Lines JR, et al. 2014. Cancer Res. 74:1924-32. Flies DB, et al. 2014. J Clin Invest. 124:1966-75. Böger C, et al. 2017. Oncoimmunology. 6:e129321.
Gene ID: 64115
UniProt: View information about VISTA on UniProt.org
Clone: W20178L
Regulatory Status: RUO
Other Names: V-Set Immunoregulatory Receptor (VSIR), V-Domain Ig Suppressor of T Cell Activation (VISTA), Stress-Induced Secreted Protein-1 (SISP1), PDCD1 Homolog, Platelet Receptor GI24 (GI24), Death Domain1alpha, DD1 alpha, PP2135, C10orf54, DD1alpha, B7-H5
Isotype: Rat IgG2a, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.