Biolegend, 607554, Ultra-LEAF™ Purified anti-human CCL23 (MPIF-1) Antibody, 1mg

Human CCL23 was initially identified in a cDNA library derived from human endothelial cells. CCL23 has six cysteine residues and possesses a separately encoded amino-terminal domain, characteristic of similar chemokines CCL6, CCL9, and CCL15. These four chemokines are weak agonists for CCR1; nevertheless, they can be proteolytically cleaved in the amino-terminus, resulting in forms with higher chemoattractant activity. Thus, NH2-terminus truncated forms of CCL23 have been detected in synovial fluid of patients with rheumatoid arthritis (RA). In vitro studies have shown that CCL23 can be processed by MMPs at the amino and carboxyl terminus. CCL23 (26-99) is the predominant stable form after using MMP1, 2, 3, 7, 8, or 12. CCL23 (30-99) is formed by MMP14, elastase, and proteolytic activity in arthritic synovial fluid. In addition, CCL23 has been detected in serum of patients with active RA, in conjunction with CXCL13, TNFSR9, M-CSF, and TNFα. High levels in serum of these cytokines correlate with joint inflammation and have been considered as biomarkers of active RA. Also, high levels of CCL23 have been detected in plasma of atherosclerotic patients. High expression of CCL23 has been found in skin of atopic dermatitis patients, and in mucosal epithelial cells and inflammatory cells in sinonasal tissues of patients with chronic rhinosinusitis (CRS).
1mg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human CCL23 (MPIF-1)
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: Neut - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by neutralizing the chemotaxis induced by recombinant Human CCL23 (MPIF-1) (Cat. No. 587004) on mouse Baf3-hCCR1 transfectant chemoattractant cells. ND50 range: 0.01 - 0.05 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2927979 (BioLegend Cat. No. 607553) AB_2927979 (BioLegend Cat. No. 607554)
Structure: Chemokine
Distribution: Monocytes, macrophages, dendritic cells, mucosal epithelial cells, and eosinophils
Function: CCL23 attracts monocytes, dendritic cells, and resting lymphocytes. CCL23 induces endothelial cell migration and angiogenesis. CCL23 inhibits colony formation of bone marrow-derived cells. IL-4 and IL-13 induce CCL23 in peripheral blood monocytes, and GM-CSF synergized with IL-4 to induce CCL23. GM-CSF and IL-5 induce CCL23 secretion from eosinophils.
Interaction: Monocytes, macrophages, epithelial cells, and dendritic cells
Ligand/Receptor: CCR1
Biology Area: Cell Biology, Signal Transduction
Molecular Family: Cytokines/Chemokines
Antigen References: Patel VP, et al. 1997. J Exp Med. 185:1163-72. Macphee CH, et al. 1998. J Immunol. 161:6273-9. Hwang J, et al. 2005. Cytokine. 30:254-63. Berahovich RD, et al. 2005. J Immunol. 174:7341-51. Novak H, et al. 2007. J Immunol. 178:4335-41. Rioja I, et al. 2008. Arthritis Rheum. 58:2257-67. Poposki JA, et al. 2011. J Allergy Clin Immunol. 128:73-81. Starr AE, et al. 2012. J Biol Chem. 287:5848-60.
Gene ID: 6368
UniProt: View information about CCL23 on UniProt.org
Clone: A15017B
Regulatory Status: RUO
Other Names: Myeloid progenitor inhibitor factor 1 (MPIF-1), Chemokine β-8 (CKβ-8), Macrophage inflammatory protein-3 (MIP-3)
Isotype: Mouse IgG1, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.