Biolegend, 607652, Purified anti-RBX1 Antibody, 100μg

RBX1 is a RING (Really Interesting New Gene) finger-like domain-containing protein. RBX1 is an E3 ubiquitin ligase component of multiple cullin-RING-based E3 ubiquitin-protein ligase (CRLs) complexes that mediate ubiquitination and subsequent proteasomal degradation of target proteins, including those involved in cell cycle progression, signal transduction, and transcription. RBX1 also promotes the neddylation of CUL1, CUL2, CUL4 and CUL4 via its interaction with UBE2M.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Full length recombinant human RBX1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This product is not recommended for IHC-P.
RRID: AB_2927959 (BioLegend Cat. No. 607651) AB_2927959 (BioLegend Cat. No. 607652)
Structure: RBX1 is a 108 amino-acid protein with a predicted molecular weight of 12 kD.
Distribution: RBX1 is ubiquitously expressed; nucleus or cytoplasm
Interaction: Part of a SCF complex that also includes CUL1, SKP1 and SKP2; part of the DCX of the DCX DET1-COP1 ubiquitin ligase complex that also potentially includes DET1, DDB1, CUL4A and COP1.
Biology Area: Cell Biology, Cell Cycle/DNA Replication, Ubiquitin/Protein Degradation
Antigen References: Hao J, et al. 2015. Oncotarget. 6:42028-39. Ohta T, et al. 1999. Molecular Cell. 3:535-41. Wertz IE, et al. 2004. Science. 303:1371-4.
Gene ID: 9978
UniProt: View information about RBX1 on UniProt.org
Clone: W19401A
Regulatory Status: RUO
Other Names: Ring-Box 1, RNF75, ROC1, Regulator of Cullins 1, RING Finger Protein 75, RING-Box Protein 1, Rbx1, E3 ubiquitin-protein ligase RBX1, E3 ubiquitin-protein ligase RBX1, Protein ZYP
Isotype: Rat IgG2a, κ