Biolegend, 607905, Alexa Fluor® 488 anti-GAPDH Antibody, 25μg

GAPDH is well known for its glycolytic function of converting D-glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate. GAPDH is a ubiquitously expressed and has a molecular mass of 36 kD. Though differentially expressed from tissue to tissue, GAPDH is frequently used as a loading control for assays involving mRNA and protein detection. In more recent studies, GAPDH has been shown to be involved in microtubule bundling, prostate cancer progression, programmed neuronal cell death, DNA replication, and DNA repair. Recent work has elucidated roles for GAPDH in apoptosis, gene expression and nuclear transport. GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer's diseases.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant protein (161-335 a.a.)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.5 - 2.0 μg/ml (1:250 - 1:1000 dilution) is recommended. It is recommended that the reagent be titrated for optimal performance for each application. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Application Notes: This clone weakly reacts with rat GAPDH.
RRID: AB_2734506 (BioLegend Cat. No. 607905) AB_2734507 (BioLegend Cat. No. 607906)
Structure: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family, predicted molecular weight 36 kD. The enzyme exists as a tetramer of identical chains.
Distribution: Ubiquitously expressed, locate in cytoplasm and perinuclear region.
Function: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family, predicted molecular weight 36 kD. The enzyme exists as a tetramer of identical chains.
Interaction: Interacts with TPPP. Interacts with EIF1AD and WARS. Interacts with SUMO4, GLUT4, nPKC-iota and CAMK2.
Biology Area: Cell Biology
Antigen References: Ercolani L, et al. 1988. J. Biol. Chem. 263:15335. Meyer-Siegler K, et al. 1991. Proc. Natl. Acad. Sci. USA. 88:8460. Hara MR, Snyder SH. 2006. Cell. Mol. Neurobiol. 26:527. Zheng L, et al. 2003. Cell. 114:255. Wang Q, et al. 2005. FASEB J. 19:869. Bae BI, et al. 2006. Proc. Natl. Acad. Sci. USA. 103:3405.
Gene ID: 5590
UniProt: View information about GAPDH on UniProt.org
Clone: W17079A
Regulatory Status: RUO
Other Names: glyceraldehyde-3-phosphate dehydrogenase
Isotype: Rat IgG2a, κ