Biolegend, 608152, Purified anti-PTK2B Antibody, 100μg

Protein-tyrosine kinase 2-beta (PTK2B or PYK2), a calcium-dependent non-receptor tyrosine kinase, belongs to focal adhesion kinase family and shares approximately 47% amino acid sequence with FAK (or PTK2). As a member of focal adhesion family, PTK2B’s phosphorylation enables interactions with other scaffold and effector proteins that regulate cell migration, adhesion, spreading, actin cytoskeleton reorganization and focal adhesion contact. PTK2B responds to several cellular stimuli to regulate critical phycological functions such as G-protein coupled receptor stimulation in vascular muscle cells, T cell and B cell antigen receptor engagement, and calcium-activated MAP kinase signaling pathway in neuronal cells. A genome-wide research suggests PTK2B correlates with Alzheimer’s disease and is able to reduce the Tau toxicity in animal models.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: E. coli derived recombinant fragment of human PTK2B
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This product does not cross react with mouse in western blotting. This product is verified to work in immunocytochemistry with methanol only fixed cells but not paraformaldehyde-fixed cells. This product is not suitable for IHC-P (fluorescence).
RRID: AB_2927961 (BioLegend Cat. No. 608151) AB_2927961 (BioLegend Cat. No. 608152)
Structure: A 1009 amino-acid protein with a predicted molecular weight of 115.8 kD; forms homodimers, or homooligomers
Distribution: Detected in cytosol and membrane; expressed preferentially in endothelium, neuronal and hematopoietic tissue
Function: A protein kinase participates in cytoskeleton rearrangement, cell spreading and cell migration; regulates humoral immune response, angiogenesis and bone remodeling
Interaction: Interacts with paxillin, RHOU, PDPK1, SRC family, and CRK-associated substrate (CAS) family
Cell Type: B cells, Basophils, Dendritic cells, Endothelial cells, Eosinophils, Lymphocytes, Monocytes, Neurons, Neutrophils, NK cells, NKT cells, T cells, Tregs
Biology Area: Cancer Biomarkers, Cell Adhesion, Cell Motility/Cytoskeleton/Structure, Neurodegeneration, Neuroscience, Signal Transduction
Molecular Family: Adaptor Proteins, Adhesion Molecules, APP/Aβ Degradation, Protein Kinases/Phosphatase, Synaptic Vesicle Trafficking/Endocytosis
Antigen References: Lev S, et al. 1995. Nature. 376:737-45. Astier A, et al. 1997. J Biol Chem. 272:228-32. Qian D, et al. 1997. J Exp Med. 185:1253-9. Lambert JC, et al. 2013. Nat Genet. 45:1452-8. Gurakt A, et al. 2019. Exp Neurol. 307:62-73. Dourlen P, et al. 2017. Mol Psychiatry. 22:874-883.
Gene ID: 2185
UniProt: View information about PTK2B on UniProt.org
Clone: W20075C
Regulatory Status: RUO
Other Names: Protein-tyrosine kinase 2-beta, FAK2, CADTK, RAFTK, PYK2, FADK2, CAK-beta, CAKB, Proline-rich tyrosine kinase 2, Related adhesion focal tyrosine kinase
Isotype: Rat IgG2b, κ