Biolegend, 608851, Purified anti-Myogenin Antibody, 25μg

Myogenin is a basic helix-loop-helix transcription factor from the myogenic regulator factor (MRF) family of transcription factors, which also includes MyoD, Myf5 and MRF4. Like other members of the MRF family myogenin is required for skeletal muscle development, and regulates the growth, repair and atrophy of skeletal muscle and myosatellite cells. In mice, loss of myogenin expression results in perinatal lethality resulting from major deficits in skeletal muscle formation. In humans, myogenin has diagnostic value as a marker for rhabdomyosarcoma and other related cancers.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic peptide containing a fragment of the N-terminus of rat myogenin
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This product was tested for use in immunocytochemistry and flow cytometry in HeLa and RD cells but did not meet passing criteria. This product was tested for use in IHC-P in human and mouse skeletal muscle tissue but did not meet passing criteria.
RRID: AB_2927974 (BioLegend Cat. No. 608851) AB_2927974 (BioLegend Cat. No. 608852)
Structure: Myogenin is a 224 amino-acid protein with a predicted molecular weight of 25.0 kd.
Distribution: Myogenin is expressed in the nucleus of skeletal muscle and myosatellite cells.
Function: Transcription factor
Interaction: Myogenin interacts with MDFI, POLR2C, SRF, SP1, and TCF3
Biology Area: Cell Biology, Transcription Factors
Antigen References: Boyer J, et al. 2014. Hum Mol Genet. 23:4249-59. Nabeshima Y, et al. 1993. Nature. 346:532-5. Zammit P. 2017. Semin Cell Dev Biol. 72:19-32
Gene ID: 4656
UniProt: View information about Myogenin on UniProt.org
Clone: F5D
Regulatory Status: RUO
Other Names: Myogenin (MYOG), Class C basic helix-loop-helix protein 3 (BHLHC3), Myogenic factor 4 (Myf-4)
Isotype: Mouse IgG1, κ