Biolegend, 608951, Purified anti-IREB2 Antibody, 25μg

IREB2 is an iron-responsive protein that regulates a cell’s levels of iron, a key micronutrient. In low-iron situations, IREB2 is free to bind to iron-responsive elements in mRNA molecules, stabilizing transferrin mRNA to increase expression of the iron uptake receptor, while blocking the translation of ferritin mRNA, a protein which sequesters excess iron molecules. However, when sufficient iron levels are present, IREB2 undergoes a conformation change to lose its mRNA binding ability, resulting in the degradation of transferrin mRNA while releasing the translation block of ferritin. Variants in the gene encoding IRP2 have been associated with lung cancer and chronic obstructive pulmonary disease.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of human IREB2
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IP, IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.0 µg/test. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This product was tested for use in ICC using PFA-Triton-X, PFA-MeOH, and MeOH only fix-perm methods, and ICFC using the Cyto-fix method, but did not meet passing criteria. This product was tested for mouse reactivity in WB and ICFC but did not meet passing criteria.
RRID: AB_2927977 (BioLegend Cat. No. 608951) AB_2927977 (BioLegend Cat. No. 608952)
Structure: IREB2 is a 953 amino-acid protein with a predicted molecular weight of 105 kD.
Distribution: IREB2 is expressed primarily in the cytoplasm
Function: IREB2 is an iron sensor
Interaction: IREB binds to iron-responsive elements in the mRNA sequences of mRNAs such as ferritin and transferrin
Ligand/Receptor: IREB2 binds iron cations
Biology Area: Chromatin Remodeling/Epigenetics
Antigen References: Papanikolaou G, et al. 2017. IUBMB Life. 69:399-413. Gozzelino R, et. al. 2016. Int J Mol. Sci. 17:130.
Gene ID: 3658
UniProt: View information about IREB2 on UniProt.org
Clone: W18260A
Regulatory Status: RUO
Other Names: Iron-responsive element-binding protein 2 (IRE-BP 2, IREB2), Iron Regulatory Protein 2 (IRP2)
Isotype: Rat IgG2a, λ