Biolegend, 609051, Purified anti-RORγt Antibody, 25μg

RORγt is a member of the nuclear hormone receptor family of transcription factors. RORγt is the isoform 2 of the RORC gene. It is produced by alternative splicing where the two 5’-exons are replaced by an alternative exon downstream in the gene, this splicing causes a shorter protein carrying three distinctive amino acids at its N-terminal end which are not present on RORγ. RORγt is mainly expressed on immature CD4+/CD8+ thymocytes and Th17 cells. It is important on lymphoid organogenesis in lymph nodes and Peyer’s patches but not on spleen. It plays an important role in promoting differentiation of pro-inflammatory Th17 cells by reducing apoptosis.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic peptide from human RORγt
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICFC, ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 1.0 µg/mL. For immunocytochemistry, a concentration range of 2.5 - 5.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody does not cross react with mouse RORγ or RORγt.
RRID: AB_2927967 (BioLegend Cat. No. 609051) AB_2927967 (BioLegend Cat. No. 609052)
Structure: RORγt is a 497 amino acid protein with a predicted molecular weight of 56 kD.
Distribution: Expressed on different subsets of T cells (mature and immature), on thymus and lymph nodes. Also, it has been found expressed on Treg cells in colon.
Function: Promotion of pro-inflammatory Th17 cells by downregulation of Fas ligand and IL2 which reduce apoptosis.
Ligand/Receptor: Ligand are cholesterol metabolites
Cell Type: Lymphocytes, Macrophages, NK cells, NKT cells, T cells, Th17, Tregs
Biology Area: Adaptive Immunity, Immuno-Oncology, Immunology, Innate Immunity, Transcription Factors
Molecular Family: Innate Immune Signaling
Antigen References: Castro G, et al. 2017. PloS One. 12: e0181868. Villey I, et al. 1999. Eur J Immunol. 29:4072-80. Lamb D, et al. 2021. Respir Res. 22:158.
Gene ID: 6097
UniProt: View information about RORgammat on UniProt.org
Clone: A21005D
Regulatory Status: RUO
Other Names: retinoic acid receptor (RAR)-related orphan receptor gamma T, ROR gamma2, RAR Related Orphan Receptor C, TOR, Retinoic Acid-Binding Receptor Gamma, IMD42, NR1f3
Isotype: Mouse IgG1, κ