Biolegend, 609352, Purified anti-CRK Antibody, 100μg

Adapter protein CRK, also known as CRK proto-oncogene adaptor protein or V-CRK avian sarcoma virus CT10 oncogene homolog, has two isoforms: CRKI (28 kD) and CRKII (40 kD). Closely associated is CRKL, or CRK-like protein (36 kD). CRKII and CRKL possess a C-terminal SH3 domain and a regulatory tyrosine phosphorylation site that are lacking in CRKI. CRK’s SH2 and SH3 domains serve as positive and negative regulators of transformation, respectively. CRK is vital to cell motility via activation of Rac1 by CRKII and CRKL and cell branching and adhesion via activation of Rap1 by CRKI. It is also vital to cell proliferation, apoptosis, formation and turnover of focal adhesions, PDGF-induced actin cytoskeleton rearrangement, cell migration, and transcription. Overexpressed in several cancers, CRK has been implicated in tumor proliferation, metastasis, and resistance to chemotherapy. This, in addition to its roles in natural killer cell activation and differentiation, lymphocyte regulation, B cell signaling, and T cell migration, makes CRK a valuable therapeutic target.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of human CRK
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P, ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: When testing by ICC, methanol rather than PFA fixation is recommended. Clone W20038C is not recommended for immunoprecipitation.
RRID: AB_2928004 (BioLegend Cat. No. 609351) AB_2928004 (BioLegend Cat. No. 609352)
Structure: CRK is a 304 amino acid protein with a predicted molecular weight of 34 kD
Distribution: Ubiquitously expressed/ Cytoplasm, Membrane
Function: Cell branching and adhesion mediated by BCAR1-CRK-RAPGEF1 signaling and activation of RAP1; proto-oncogene
Interaction: Rac1, Rap1, p130Cas, paxillin, Dock180, C3G/GRF2, ZAP70, Gab3, Cbl-b, HPK1, PI3K, PDGFRA, PDGFRB, IRS4, MAPK8, MAP4KI
Biology Area: Cell Biology
Molecular Family: Adaptor Proteins, Adhesion Molecules, Scaffold Proteins
Antigen References: Antoku S and Mayer BJ. 2009. J Cell Sci. 122:4228-38. Liu, D. 2014. Immunol Cell Biol. 92:80-9. Park T. 2021. Cells. 10:739.
Gene ID: 1398
UniProt: View information about CRK on UniProt.org
Clone: W20038C
Regulatory Status: RUO
Other Names: CRK, CRKII, p38, V-CRK Avian Aarcoma Virus CT10 Oncogene Homolog, CRK proto-oncogene adaptor protein
Isotype: Rat IgG2a, κ