Biolegend, 609451, Purified anti-SMYD2 Antibody, 25μg

SET and MYND domain-containing protein 2, also known as SMYD2, is one of five members of the SMYD family of lysine methyltransferases composed of an evolutionarily conserved SET domain and a zinc finger-containing MYND domain. SMYD2 localizes to the nucleus and cytoplasm and methylates histones such as H2B, H3K4, H3K36, and H4 as well as non-histone substrates, notably p53 on lysine 370. As a protein lysine methyltransferase, SMYD2 is a regulator of transcription and epigenetic signaling and functions in the regulation of cell cycle and apoptosis. It has also been shown to promote NF-κB signaling pathways via methylation of TRAF2 and STAT3. Aberrant SMYD2 expression is associated with a variety of cancers and other diseases including kidney disease, cardiovascular disease, and rheumatoid arthritis. It promotes tumorigenesis and metastasis through the repression of tumor suppressor proteins RB1, p53, and PTEN.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human SMYD2 protein fragments
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested WB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For western blotting, the suggested use of this reagent is 0.25 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone 1G10-E11-H9 is not recommended for testing in mouse.
RRID: AB_2928003 (BioLegend Cat. No. 609451) AB_2928003 (BioLegend Cat. No. 609452)
Structure: SMYD2 is a 433 amino acid protein with a predicted molecular weight of 50 kD
Distribution: Ubiquitously expressed/Cytoplasm, Nucleus
Function: Chromatin regulator, Methyltransferase,Transcription
Interaction: P53, RB1, H2B, H4, H3K36, H2K4, HSP90/p23, PTEN, ERα, ALK, PARP1, MAPKAPK3, STAT3
Ligand/Receptor: Metal-binding, S-adenosyl-L-methionine, Zinc
Biology Area: Cell Biology
Molecular Family: Adaptor Proteins, Adhesion Molecules, Scaffold Proteins
Antigen References: Abu-Farha M, et al. 2008. Mol Cell Proteomics. 7:560-72. Ferguson AD, et al. 2011. Structure. 19:1262-73. Wu W, et al. 2021. Clin Transl Med. 11:e591. Yi X, et al. 2019. Clin Epigenetics. 11:112.
Gene ID: 56950
UniProt: View information about SMYD2 on UniProt.org
Clone: 1G10-E11-H9
Regulatory Status: RUO
Other Names: SET and MYND domain-containing protein 2, Histone methyltransferase SMYD2, HSKM-B, Lysine N-methyltransferase 3C, N-lysine methyltransferase SMYD2, ZMYND14, KMT3C
Isotype: Mouse IgG2b, κ