Biolegend, 609551, Purified anti-Oligodendrocyte Marker O4 Antibody, 25μg

Oligodendrocytes are the myelinating cells of the central nervous system (CNS). Similar to the Schwann cells in the peripheral nervous system (PNS), their function is to provide support and isolation to neuronal axons. Their importance has been denoted on de-myelinating diseases such as multiple sclerosis, amyotrophic lateral sclerosis, periventricular leukomalacia, and also, they have been associated with dementia, schizophrenia, cognitive impairment, and bipolar disorder. Oligodendrocytes develop during embryogenesis; however, a small proportion of progenitor stem cells are present in the adult brain. Thus, three stages of differentiation can be identified in the cerebral white matter, and specific markers have been associated which each particular stage. Sulfated Galactocerebroside O4 (marker O4) is a major galactosphingolipid component of oligodendrocyte plasma membrane. It is produced from early immature non-myelinating oligodendrocytes to mature myelinating oligodendrocytes, and together with expression of other markers, it has been widely used as characteristic marker for these specific differentiation stages.
25μg
Verified Reactivity: Human, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: White matter from bovine corpus callosum (from developer)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested ICC, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry on testis samples, a concentration range of 1.25 - 5.0 µg/mL is suggested. For immunohistochemistry on cerebellum samples, a concentration range of 0.5 - 1.25 µg/mL is suggested. For immunocytochemistry, a concentration range of 2.5 - 5.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone O4 recognizes specifically O4 antigen by immunocytochemistry in oligodendrocytes derived from cortical stem cells using different fixation/permeabilization methods such as methanol, PFA/Triton X-100, and PFA/methanol, where the last method shows the highest signal intensity. Following immunohistochemistry in paraffin sections, clone O4 showed specific high staining on cerebellum white matter, and very low to none staining in the molecular layer at the recommended concentration. By flow cytometry staining on differentiated cortical stem cells, clone O4 stained at high intensity complex cells (oligodendrocytes) compared with low complexity cells (undifferentiated progenitor stem cells).
RRID: AB_2927990 (BioLegend Cat. No. 609551) AB_2927990 (BioLegend Cat. No. 609552)
Structure: It is a sulfated galactocerebroside (glycosphingolipid), composed of a single galactose linked to a ceramide
Distribution: Present in the plasma membrane of early immature to mature oligodendrocytes
Cell Type: Neural Stem Cells, Oligodendrocytes
Biology Area: Neurodegeneration, Neuroscience, Neuroscience Cell Markers
Antigen References: Bansal R, et al. 1989. J Neurosci Res. 24:548-57. Bansal R, et al. 1999. J Neurosci. 19:7913-24. Ahmad I, et al. 2021. PloS One. 16:e0256155. Rasti BF, et al. 2020. Int J Nanomedicine. 15:3903-3920.
Gene ID: NA
UniProt: View information about Oligodendrocyte Marker O4 on UniProt.org
Clone: O4
Regulatory Status: RUO
Other Names: Galactocerebroside O4, Oligodendrocyte marker O4, Oligodendrocyte O4 antigen, Sulfated Galactocerebroside O4
Isotype: Mouse IgM, κ