Biolegend, 609701, Purified anti-SSRP1 Antibody, 25μg

SSRP1 is a nuclear protein and a component of the FACT complex (facilitates chromatin transcription). The FACT complex is composed of the SUPT16H (FACT140) and the SSRP1 FACTp80 proteins. This complex interacts with nucleosomes and histone H2A/H2B dimers to promote nucleosome disassembly and allow transcription elongation. This protein interacts with dsDNA. The SSRP1 protein has been shown to be modified by cisplatin and may protect DNA from repair and block DNA replication. The 10D1 monoclonal antibody recognizes the human SSRP1 protein and has been shown to be useful for Western blotting.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant (partial) (308-524aa)
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, ChIP - Verified IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg per 5 ml antibody dilution buffer for each mini-gel. For ChIP applications, the suggested dilution is 1:50-1:200 by volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: 10D1 displayed a stronger affinity for SSRP1 than BioLegend clone 3E4 when tested for western blot application.
Application References(PubMed link indicates BioLegend citation): Tan BC, et al. 2006. EMBO 25:3975. (ChIP) Carvalho S, et al. 2013. Nucleic Acids Res. 41:2881. PubMed Tan BC, et al. 2004. J. Biol. Chem. 279:9321. (Immunogen) Lolis AA, et al. 2013. J Biol Chem. 288:7676. PubMed Aida M, et al. 2013. PNAS. 110:7784. PubMed Dermawan JK, et al. 2014. Mol Cancer Ther. 13:2203. PubMed Shakya A, et al. 2015. Mol Cell Biol. 35:1014. PubMed
Product Citations: Goswami I, et al. 2022. EMBO Rep. 23:e53684. PubMed Jeong E, et al. 2022. Autophagy. 18:2333. PubMed Dermawan J, et al. 2016. Cancer Res . 76: 2432-2442. PubMed Srinivasan R, et al. 2020. Sci Adv. 6:eaaz9115. PubMed Fleyshman D, et al. 2017. Oncotarget. 8:20525. PubMed Isa NF, et al. 2021. Vaccines (Basel). 9:. PubMed Safina A, et al. 2017. Nucleic Acids Res. 10.1093/nar/gkw1366. PubMed Carvalho S, et al. 2013. Nucleic Acids Res. 41:2881. PubMed Shakya A, et al. 2015. Mol Cell Biol. 35:1014. PubMed Wever V, et al. 2012. PLoS One. 7:e39510. PubMed Li X, et al. 2021. J Biol Chem. 297:101360. PubMed Narain A, et al. 2021. Molecular Cell. 81(15):3110-3127.e14. PubMed Sundarraj J, et al. 2021. Wellcome Open Res. 2:83. PubMed Lolis A, et al. 2013. J Biol Chem. 288:7676. PubMed Piquet S et al. 2018. Molecular cell. 72(5):888-901 . PubMed Aida M, et al. 2013. Proc Natl Acad Sci U S A. 110:7784. PubMed Dermawan J, et al. 2014. Mol Cancer Ther. 13:2203. PubMed Sandlesh P, et al. 2018. PLoS One. 15:81. PubMed Ali A, et al. 2019. FASEB J. 33:2621. PubMed Prendergast L, et al. 2020. Oncogene. 39:5124. PubMed Paul S, et al. 2018. J Biol Chem. 293:19303. PubMed Wang AS, et al. 2020. Molecular Cell. 79(2):221-233.e5.. PubMed
RRID: AB_315730 (BioLegend Cat. No. 609701) AB_315731 (BioLegend Cat. No. 609702)
Structure: SSRP family, HMG box domain; 81 kD
Distribution: Nuclear
Function: Interacts with nucleosomes and histone H2A/H2B dimers to promote nucleosome disassembly and allow transcription elongation, binds to dsDNA. Modified by cisplatin, may protect DNA from repair and block DNA replication
Interaction: Component of FACT ATP-dependent chromatin remodeling complex, SUPT16H/Cdc68 in FACT complex, H2A/H2B dimers
Biology Area: Cell Biology, Chromatin Remodeling/Epigenetics, DNA Repair/Replication, Transcription Factors
Antigen References: 1. LeRoy G, et al. 1998. Science 282:1836. 2. Orphanides G, et al. 1998. Cell 92:105. 3. Orphanides G, et al. 1999. Nature 400:284.
Gene ID: 6749
UniProt: View information about SSRP1 on UniProt.org
Clone: 10D1
Regulatory Status: RUO
Other Names: Structure specific recognition protein 1 (SSRP1), Facilitates Chromatin Transcription 80 kD subunit, FACTp80, Recombination signal sequence recognition protein
Isotype: Mouse IgG2b, κ