Biolegend, 610301, Purified anti-mTOR Phospho (Ser2448) Antibody, 25μg

mTOR is a member of the PI3 family of kinases and plays a critical role in regulating cell proliferation and metabolism in response to nutrient availability, cellular energy status, hormones, growth factors, and stress. mTOR is found as a component of two functionally distinct oligomeric complexes, mTORC1 and 2 (mTOR complex 1 and 2). mTORC1 coordinates nutrient availability with cell growth and proliferation through phosphorylation of upstream regulators of proliferation and ribosomal biogenesis including eIF4BP1 and RPS6KB1 and RPS6KB2. Additionally, mTORC1 contols autophagy through negative regulation of an autophagy-promoting kinase complex comprised of ULK1, ATG13, and FIP200. mTORC2 regulates proliferation and metabolism via phosphorylation of IG-1R, InsR, and Akt, and also plays a role in cytoskeletal architecture and reorganization through modification of Rac1. The mTOR subunit of mTORC1 and 2 is phosphorylated at Ser2448 in a PI3/Akt- signaling-dependent manner; this post-translational modification is associated with increased mTORC activity.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. For immunocytochemistry, a concentration of 5 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Based off complete sequence homology of the immunizing peptide, this clone is predicted to recognize both mouse and rat mTOR phosphorylated at serine 2448. NIH/3T3 cells (murine origin) used in this experiment contain less mTOR than the human-derived HeLa cells (see pan mTOR blot). Thus, the reduced signal observed in these lysates is due to less total mTOR and not a decreased affinity of this clone for mouse mTOR phosphor (Ser2448).
Product Citations: Rahmani-Kukia N, et al. 2023. Cell J. 470:25. PubMed Chen Z, et al. 2021. J Exp Med. 218:. PubMed
RRID: AB_2801105 (BioLegend Cat. No. 610301) AB_2801106 (BioLegend Cat. No. 610302)
Structure: mTOR is a 2,549 amino acid protein with a predicted molecular weight of ~289 kD.
Distribution: Ubiquitously expressed; cytsosolic, lysosomal, and nuclear cellular localization
Function: Regulates cell proliferation and metabolism
Interaction: Part of the mammalian target of rapamycin complex 1 (mTORC1) which contains MTOR, MLST8, RPTOR, AKT1S1/PRAS40, and DEPTOR; also part of the mammalian target of rapamycin complex 2 (mTORC2) which contains MTOR, MLST8, PRR5, RICTOR, MAPKAP1, and DEPTOR.
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Cell Proliferation and Viability, Protein Synthesis, Signal Transduction
Molecular Family: Phospho-Proteins, Protein Kinases/Phosphatase
Antigen References: Brown EJ, et al. 1994. Nature. 369:756-8. Dennis P, et al. 2001. Science. 294:1102. Fingar D, et al. 2002. Genes Dev. 16:1472. Nojima H, et al. 2003. J. Biol. Chem. 278:15461.
Gene ID: 2475
UniProt: View information about mTOR Phospho Ser2448 on UniProt.org
Clone: A17024A
Regulatory Status: RUO
Other Names: Mammalian target of rapamycin, FRAP, RAFT
Isotype: Mouse IgG1, κ