Biolegend, 610601, Purified anti-MEK1 Phospho (Ser298) Antibody, 25μg

MEK1 and MEK2 (Map/Erk Kinase) are serine/threonine kinases in the MAPK/ERK signaling axis that play a critical role in cellular proliferation, differentiation, and migration. Phosphorylation at MEK1 (Ser298) by PAK1 enhances the ability of upstream Raf kinases to phosphorylate regulatory serines (Ser218 and Ser222) in the activation loop, thereby stimulating kinase activity.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human MEK1 peptide phosphorylated at Ser298. Complete Freund’s adjuvant.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.0625 - 1.0 µg per mL. For flow cytometry using our True-Phos™ Perm Buffer, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2810657 (BioLegend Cat. No. 610601) AB_2810658 (BioLegend Cat. No. 610602)
Structure: MEK1 is a 393 amino acid protein with a predicted molecular weight of 43 kD
Distribution: Ubiquitously expressed/cytosolic
Biology Area: Cell Biology, Cell Proliferation and Viability, Signal Transduction
Molecular Family: Phospho-Proteins, Protein Kinases/Phosphatase
Antigen References: Song C, et al. 2017. Sci Rep. 7:46833. Jeong DE, et al. 2017. EMBO J. 36:1046. Kyriakakis E, et al. 2017. Cell Signal. 35:163. Narbonne P, et al. PLoS Genet. 13:e1006738. Wu H, et al. 2013. PLoS One. 8:e83737. Wang Z, et al. 2010. Oncogene. 29:3362. Moniz S, et al. 2007. Oncogene. 26:6071. Park ER, et al. 2007. Cell Signal. 7:1488.
Gene ID: 5604
UniProt: View information about MEK1 Phospho Ser298 on UniProt.org
Clone: A16117B
Regulatory Status: RUO
Other Names: MEK1, MAP2K1, CFC3, MKK1, MAPKK1, PRKMK1
Isotype: Mouse IgG1, κ