Biolegend, 610752, Purified anti-Serpin E1 Antibody, 100μg

Serpin E1 is a member of the serine proteinase inhibitors (serpin) superfamily, which is divided into two groups: inhibitory and non- inhibitory serpins. Serpin E1 is the most important physiological inhibitor of tissue-type plasminogen activator (t-PA) and urokinase-type plasminogen activator (u-PA), and therefore is a key regulator of fibrinolysis via inhibition of the activation of the broad-spectrum serine protease plasmin. Serpin E1 forms a covalent bond with both t-PA and u-PA (1:1 molar ratio). It is known that several receptors (uPAR, LRP-1A, megalin, VLDLR, and LRP-1B) bind and mediate endocytosis of plasminogen activator-Serpin E1 complexes. Serpin E1 is present in blood and the extracellular matrix in a complex with vitronectin. Elevated Serpin E1 levels have been implicated in the pathogenesis of atherosclerosis and myocardial infarction (MI). High levels of plasma Serpin E1 in patients precede MI. Abnormal levels of Serpin E1 is also associated with metabolic syndrome, obesity, obstructive nephropathy, cancer, cardiac fibrosis, pulmonary fibrosis, and bleeding thrombosis.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human Serpin E1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality testedWB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone A16081E is not suitable for ICC.
RRID: AB_2936732 (BioLegend Cat. No. 610751) AB_2936732 (BioLegend Cat. No. 610752)
Structure: Serpin E1 is a 402 amino-acid protein with a predicted molecular weight of 45 kD.
Distribution: Cytoplasmic, secreted
Function: Serpins bind to and inhibit proteinases
Interaction: Serpin E1 binds to and inhibits urokinase and tissue plasminogen activator.
Biology Area: Cardiovascular Biology, Cell Adhesion, Inhibitory Molecules
Antigen References: van Mourik JA, et al. 1984. J Biol Chem. 259:14914-21. Zhou A, et al. 2003. Nat Struct Biol. 10:541-4. Tsantes AE, et al. 2008. Thromb Res. 122:736-42. Dupon DM, et al. 2009. Front Biosciences 14:1337-61. Ploplis VA. 2011. Curr Drug Targets. 12:1782-9. Yasar Yildiz S, et al. 2014. Scientific World Journal. 2014:858293.
Gene ID: 5054
UniProt: View information about Serpin E1 on UniProt.org
Clone: A16081E
Regulatory Status: RUO
Other Names: Plasminogen activator inhibitor type 1 (PAI-1, PLANH1), Serine (Or Cysteine) Proteinase Inhibitor, Endothelial Plasminogen Activator Inhibitor
Isotype: Mouse IgG2b, κ