Biolegend, 611152, Purified anti-Peroxiredoxin 1 Antibody, 100μg

Peroxiredoxin 1, also called PRDX1, is one of six members of the superfamily of thiol-dependent peroxidases. Peroxiredoxin 1 can eliminate reactive oxygen and nitrogen species. As a critical antioxidant enzyme, it utilizes an N-terminal cystine to detoxify peroxides and protect cells from oxidative stress damage. As a sensor of inflammation response, extracellular Peroxiredoxin 1 is released to trigger TLR4-dependent secretion of TNF-α and IL-6 in macrophage and dendritic cell maturation. Peroxiredoxin 1 is increased in bone marrow-derived macrophages by lipopolysaccharide (LPS) and IFN-γ exposure. In microglia, the expression level of Peroxiredoxin 1 is regulated through ROS/p38/MAPK pathway and protects microglia from cell death. Overexpression of Peroxiredoxin 1 in rat primary hippocampus neuronal and PC12 cells promotes survival under amyloid β toxicity. In addition to its role as an antioxidant, oxidized Peroxiredoxin 1 forms an oligomer that acts as a chaperone to suppress the c-Myc oncogene and promotes tumor cell apoptosis.
100μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant protein of human Peroxiredoxin 1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. For immunocytochemistry, a concentration of 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This product is not recommended for use in IHC-P. For immunocytochemistry, fixation with ice-cold methanol is recommended.
RRID: AB_2936735 (BioLegend Cat. No. 611151) AB_2936735 (BioLegend Cat. No. 611152)
Structure: Peroxiredoxin 1 is a 199 amino acid protein with an expected molecular weight of 22 kD. It exists as a homodimer and homodecamer.
Distribution: Mainly expressed in cytoplasm; Distributed ubiquitously across tissues
Function: Catalyzes the reduction of hydrogen peroxide and organic hydroperoxides
Interaction: Peroxiredoxin 1, PTEN, Androgen receptor, C-ABL
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Death, Signal Transduction, Transcription Factors
Antigen References: Shau H, et al. 1993. Cell Immunol. 147:1-11. Wood ZA, et al. 2003. Trends Biochem. Sci. 28:32-40. Cumming RC, et al. 2007. J Biol Chem. 282:30523-34. Kim SU, et al. 2008. Biol Pharm Bull. 31:820-5.
Gene ID: 5052
UniProt: View information about Peroxiredoxin 1 on UniProt.org
Clone: 6A6-4H3-7A3
Regulatory Status: RUO
Other Names: PRDX1, Natural killer cell-enhancing factor A, NKEF-A, Proliferation-associated gene protein, PAG, Thioredoxin peroxidase 2, Thioredoxin-dependent peroxide reductase 2, Thioredoxin-dependent peroxiredoxin 1, PAGA, PAGB, TDPX2
Isotype: Mouse IgG1, κ