Biolegend, 611352, Purified anti-NF-κB p65 Antibody, 100μg

NF-κB p65, also known as NFKB3 and RelA, is one of five members of the nuclear factor kB (NF-kB)/Rel family. Inactive NF-κB p65 is sequestered in the cytoplasm as a complex with IκB inhibitory proteins. Upon activation of the NF-κB pathway, IκB proteins are degraded, thus releasing NF-κB p65 to the nucleus. Binding of NF-κB p65 to transcriptional cofactors is induced via phosphorylation on serines 276, 529, 536, and 471. As a transcription factor, NF-κB p65 is vital to cell proliferation, immune response, survival, and apoptosis. Through regulation of chondrocyte and osteoblast differentiation and survival, NF-κB p65 has a crucial role in skeletal development. Overexpressed in several cancers, NF-κB p65 functions as an oncogene and has been implicated in tumorigenesis, metastasis, tumor angiogenesis, and chemoresistance.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic peptide corresponding to human NF-κB p65
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P, ICC, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 0.50 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application
Application Notes: When testing by ICC, we recommend fixation with 4% PFA followed by permeabilization with Triton X-100 or ice-cold methanol.
RRID: AB_2936734 (BioLegend Cat. No. 611351) AB_2936734 (BioLegend Cat. No. 611352)
Structure: NF-κB p65 is a 551 amino acid protein with a predicted molecular weight of 60 kD.
Distribution: Ubiquitously expressed/ Cytoplasm and Nucleus
Function: Transcription, RAS pathway, host-virus interaction, oncogene
Interaction: NF-kappa-B, TLE5, TLE1, TP53BP2, GSK3B, MAP3K14, HDAC3, AKIP1, FOXP3
Biology Area: Cell Biology, Immuno-Oncology, Immunology, Innate Immunity, Transcription Factors
Molecular Family: Phospho-Proteins
Antigen References: Giridharan S and Srinivasan M. 2018. J Inflamm Res. 11:407-419. Ren C, et al. 2021. Cell Death Differ. 29:381-392. Yu S, et al. 2020. FEBS Lett. 594:1368-1378.
Gene ID: NA
UniProt: View information about NF-kB p65 on UniProt.org
Clone: A21012A
Regulatory Status: RUO
Other Names: RelA, P65, NFKB3, Nuclear Factor NF-Kappa-B P65 subunit, Transcription Factor P65, NF-Kappa-B Transcription Factor P65, RELA Proto-Oncogene NF-KB subunit
Isotype: Mouse IgG1, κ