Biolegend, 611552, Purified anti-human ALK Antibody, 100μg

ALK is expressed widely in the central nervous system, where it plays important roles in neurogenesis and the development of the nervous system. ALK is a receptor tyrosine kinase that phosphorylates tyrosines on target proteins upon ligand binding (known ligands include pleiotrophin, midkine, FAM150A, and FAM150B). ALK was originally discovered in anaplastic large-cell lymphoma cells where a chromosomal translocation leads to constitutive activation of the ALK kinase domain, making ALK a target of inhibition in cancer therapies.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of human ALK
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: W16189A is not suitable for ICC, FC, and IHC-P. W16189A is weakly cross-reactive with mouse ALK in WB.
RRID: AB_2941602 (BioLegend Cat. No. 611551) AB_2941602 (BioLegend Cat. No. 611552)
Structure: ALK is a 1,620 amino acid protein with a predicted molecular weight of 176.4 kD.
Distribution: ALK is expressed in the plasma membrane and is expressed in many neuronal subtypes in specific regions of the central and peripheral nervous system.
Function: Upon ligand binding ALK dimerizes and autophosphorylates, gaining kinase activity
Interaction: ALK phosphorylates members of the MAPK and NF-KB signaling pathways
Ligand/Receptor: ALK activation can proceed via binding of pleiotrophin, midkine, FAM150A or FAM150B
Cell Type: Neurons
Biology Area: Cell Proliferation and Viability
Molecular Family: CD Molecules, Protein Kinases/Phosphatase
Antigen References: Souttou B, et al. 2001. J Biol Chem. 276:9526-31. Hallberg B, et al. 2016. Ann Oncol. 27 Suppl 3:iii4-iii15.
Gene ID: 238
UniProt: View information about ALK on UniProt.org
Clone: W16190A
Regulatory Status: RUO
Other Names: Anaplastic lymphoma kinase (ALK), ALK tyrosine kinase receptor, cluster of differentiation 246 (CD246), Mutant Anaplastic Lymphoma Kinase, NBLST3
Isotype: Rat IgG2a, κ