Biolegend, 612160, Purified anti-human MMP-8 Antibody, 100μg

MMP-8, also termed neutrophil collagenase, is a member of the matrix metalloproteinase family proteins (MMPs). Members of this family are structurally related, zinc-containing enzymes that degrade the extracellular matrix (ECM) and connective tissue proteins. MMP-8 consists of a prodomain that is cleaved upon activation, a catalytic domain containing the zinc binding site, a hinge region, and a carboxyl terminal domain with hemopexin-like repeats. Substrates of MMP-8 include type I, II, and III triple-helical collagens, gelatin peptides, proteoglycans, fibronectin, aggrecan, substance P, serpins, β-casein, and angiotensin. MMP-8 is released by neutrophils upon IL-1β IL-8, GM-CSF and TNF-α stimulation. Besides playing a role in phagocytosis, MMP-8 has been implicated in rheumatoid arthritis (RA) because of its high efficiency in infiltrating connective tissue and breaking down ECM. In fact, serum level of MMP-8 is a strong predictor of mortality in RA, as this is consistent with increased activation of neutrophils in RA. In addition, MMP-8 has been shown to play a crucial role in cardiovascular diseases by promoting atherosclerotic lesion formation. This is supported by the finding that MMP-8 knockout mice have less endothelial cells or plaque angiogenesis in the atherosclerotic plaques. In a clinical study, it was shown that the ratio of MMP-9/TIMP-1, as well as MMP-8 serum levels, could be a useful predictor of prognosis in patients with hepatocellular carcinomas.
100μg
Verified Reactivity: Human
Antibody Type: Polyclonal
Host Species: Goat
Immunogen: Recombinant Human MMP-8 (carrier-free)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: Direct ELISA - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.
Additional Product Notes: This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (clone Poly23A11) could function as a capture and detection antibody as a self-pairing antibody and using Recombinant Human MMP-8 (carrier-free) protein (Cat. Nos. 556102 and 556104) as the assay standard. For assay development flexibility purposes, this product is provided as a purified format.
RRID: AB_3720587 (BioLegend Cat. No. 612160)
Structure: Monomer
Distribution: Neutrophils, macrophages, monocytes
Function: MMP8 degrades ECM proteins (collagen type I, II, and III, gelatin, proteoglycans, fibronectin, aggrecan, substance P, serpins, β-casein, and angiotensin); IL-1β and IL-6 stimulation activates neutrophils to release MMP-8.
Cell Type: Macrophages, Monocytes, Neutrophils
Biology Area: Angiogenesis, Cell Adhesion, Cell Biology, Neuroinflammation, Neuroscience
Molecular Family: Enzymes and Regulators
Antigen References: Nagase H, and Woessner JF Jr. 1999. J. Biol. Chem. 274:21491. Gill SE and Parks WC. 2008. Int. J. Biochem. Cell Biol. 40:1334. Mattey DL, et al. 2012. Arthritis Res. Ther. 14:R204. Fang C. et al. 2013. Cardiovasc. res. 99:146. Lempinen M. et al. 2013. Ann. Med. 45:482. Sommer K, et al. 2013. PLos One. 8:e73992.
Gene ID: 4317
UniProt: View information about MMP-8 on UniProt.org
Clone: Poly23A11
Regulatory Status: RUO
Other Names: Matrix metalloproteinase (MMP-8), PMNL collagenase (PMNL-CL), Neutrophil Collagenase
Isotype: Goat Polyclonal IgG