Biolegend, 612161, Purified anti-human MMP-7 Antibody, 100μg

Matrix metalloproteinases (MMPs) are a family of zinc-dependent endopeptidases that degrade components of the extracellular matrix (ECM) and play essential roles in various physiological and pathological processes such as morphogenesis, differentiation, angiogenesis, tissue remodeling, and tumor invasion. A typical MMP consists of a pro-peptide of about 80 amino acids, a catalytic metalloproteinase domain of about 170 amino acids, a linker peptide of variable lengths and a hemopexin domain of about 200 amino acids. The zinc binding motif HEXXHXXGXXH in the catalytic domain, and the ‘‘cysteine switch’’ motif PRCGXPD in the pro-peptide are common structural signatures. MMP-7, Matrilysin, is one of the smallest members of the MMP family consisting of a pro-peptide domain and a catalytic domain. It can degrade casein, laminin, fibronectin, collagen type III/IV/V/IX/X/XI, gelatin type I/II/IV/ V, elastin, and proteoglycans. MMP-7 is secreted specifically by epithelial cells and its over-expression has been observed in many tumor types such as colorectal cancer, epidermolysisbullosa associated skin cancer, bladder cancer, gastric cancers, pancreatic cancer and esophageal cancer. This enzyme serves essential functions in both innate defense and wound healing, and appears to be one of the most important MMPs in human colon cancers. It has been reported that MMP-7 contributes to tumor malignancy probably by cleaving cell surface proteins such as Fas ligand (reducing its effectiveness in triggering Fas-mediated apoptosis) and E-cadherin (disruption to the E-cadherin/beta-catenin complex results in a switch to a more invasive phenotype in cancer cells). In addition, degradation of IgG by MMP-7 may protect tumor cells from the immune system, since cleavage of IgG will remove much of the immunoglobulins' functionality.
100μg
Verified Reactivity: Human
Antibody Type: Polyclonal
Host Species: Goat
Immunogen: Recombinant Human MMP-7 (carrier-free)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: Direct ELISA - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.
Additional Product Notes: This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (clone Poly23A29) could function as a capture and detection antibody as a self-pairing antibody and using Recombinant Human MMP-7 (carrier-free) protein (Cat. Nos. 761302, 761304 and 761306) as the assay standard. For assay development flexibility purposes, this product is provided as a purified format.
RRID: AB_3720588 (BioLegend Cat. No. 612161)
Structure: Monomer
Distribution: Neutrophils, macrophages, monocytes
Function: ECM remodeling and wound healing; TIMPs are known inhibitor of MMP-7.
Interaction: Extracellular Matrix (ECM)
Ligand/Receptor: TIMP-1, TIMP-2, TIMP-4
Biology Area: Angiogenesis, Cell Adhesion, Cell Biology, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family: Enzymes and Regulators
Antigen References: Basu S, et al. 2015. PLoS One. 10:e0123979. Tallant C, et al. 2010. Biochim. Biophys. Acta. 1803:20. Nagase H, et al. 2006. Cardiovasc. Res. 69:562. Rundhaug JE. 2005. J. Cell. Mol. Med. 9:267. Mott JD, Werb Z. 2004. Curr. Opin. Cell. Biol. 16:558. Kioi M, et al. 2003. Oncogene. 22(54):8662-70.
Gene ID: 4316
UniProt: View information about MMP-7 on UniProt.org
Clone: Poly23A29
Regulatory Status: RUO
Other Names: Matrix metalloproteinase 7 (MMP-7), MMP, Matrilysin, Pump-1 protease, Uterine metallo
Isotype: Goat Polyclonal IgG