Biolegend, 612310, Alexa Fluor® 647 anti-Cytochrome c Antibody, 100μg

Cytochrome c is a 15 kD protein found in the mitochondrial intermembrane space with a heme-binding domain. Cytochrome c is a component of the electron transport chain; the heme group transfers electrons from cytochrome b-c1 complex to cytochrome oxidase complex. Cytochrome c initiates apoptosis by release to cytoplasm and binding Apaf-1 which activates procaspase 9. Cytochrome c interacts with the cytochrome b-c1 complex, cytochrome oxidase complex, heme, Apaf-1, and Caspase 9 proteins. The 6H2.B4 monoclonal antibody recognizes human, mouse, and rat cytochrome-c and has been shown to be useful for intracellular flow cytometric staining, Western blotting, immunoprecipitation, and immunofluorescence staining.
100μg
Verified Reactivity: Mouse, Rat, Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Rat cyt c-OVA
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. For immunocytochemistry, a concentration range of 2.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: intracellular flow cytometry5, immunofluorescence microscopy3,5, immunoprecipitation4, and immunocytochemistry5.
Application References(PubMed link indicates BioLegend citation): Goshorn SC, et al. 1991. J. Biol. Chem. 266:2134. Jemmerson R, et al. 1991. Eur. J. Immunol. 21:143. Chandra D, et al. 2002. J. Biol. Chem. 277:50842. (IF) Semenkova L, et al. 2003. Eur. J. Biochem. 270:4388. (IP) Shih S-F, et al. 2001. J. Biol. Chem. 276:21870. (ICFC ICC IF) She P, et al. 2011. Am J. Physiol Endcorinol Metab.301:E49. PubMed McGuire, KA., et al. 2011. J. Virol 85:10806. PubMed
Product Citations: Carter BZ, et al. 2023. Blood Cancer J. 13:57. PubMed Hemming ML, et al. 2022. Clin Cancer Res. 28:2397. PubMed Xu Y, et al. 2023. EMBO Rep. 24:e55503. PubMed Manzano-Muñoz A, et al. 2022. NPJ Precis Oncol. 6:90. PubMed Pan R, et al. 2022. Cell. 185:1521. PubMed Pogacar Z, et al. 2022. Life Sci Alliance. 5:. PubMed Alcon C, et al. 2020. Cell Death Dis. 0.898611111. PubMed Sekar G, et al. 2022. iScience. 25:105064. PubMed Ramsey HE, et al. 2018. Cancer Discov. 8:1566. PubMed Deng J, et al. 2021. Cell Death Dis. 12:978. PubMed Meyer LK, et al. 2019. J Clin Invest. 130:863. PubMed Olesinski EA, et al. 2021. STAR Protocols. 2(2):100461. PubMed Fraser C, et al. 2022. Nat Commun. 13:5789. PubMed Bhatt S, et al. 2020. Cancer Cell. 38(6):872-890.e6. PubMed Jenson J, et al. 2017. Elife. 10.7554/eLife.25541. PubMed Canedo EC, et al. 2021. JCI Insight. 6:. PubMed Potter DS, et al. 2021. Cell Death Dis. 12:741. PubMed Manzano-Muñoz A, et al. 2021. Front Cell Dev Biol. 9:695225. PubMed Victorino F, et al. 2021. eLife. 0.416666666666667. PubMed Alcon C, et al. 2021. Cells. 10:. PubMed Dhimolea E, et al. 2021. Cancer Cell. 39:240. PubMed Liang Z, et al. 2021. J Immunol. 207:2039. PubMed
RRID: AB_2565241 (BioLegend Cat. No. 612310)
Structure: Heme binding domain; 15 kD
Distribution: Mitochondrial intermembrane space
Function: Component of electron transport chain; heme group transfers electrons from cytochrome b-c1 complex to cytochrome oxidase complex. Initiates apoptosis by release to cytoplasm and binding Apaf-1 which activates procaspase 9
Interaction: Cytochrome b-c1 complex, cytochrome oxidase complex, heme, Apaf-1, Casp9
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Mitochondrial Function, Neuroscience, Neuroscience Cell Markers
Molecular Family: Mitochondrial Markers
Antigen References: 1. Liu X, et al. 1996. Cell. 86:147. 2. Li P, et al. 1997. Cell. 91:479. 3. Zhang Z, et al. 2003. Gene 312:61. 4. Ferguson H, et al. 2003. J. Biol. Chem. 278:45793.
Gene ID: 1355
UniProt: View information about Cytochrome c on UniProt.org
Clone: 6H2.B4
Regulatory Status: RUO
Other Names: Cyt c
Isotype: Mouse IgG1, κ