Biolegend, 612503, Purified anti-Cytochrome c Antibody, 50microl

Cytochrome c is a 15 kD protein found in the mitochondrial intermembrane space with a heme-binding domain. Cytochrome c is a component of the electron transport chain; the heme group transfers electrons from cytochrome b-c1 complex to cytochrome oxidase complex. Cytochrome c initiates apoptosis by release to cytoplasm and binding Apaf-1 which activates procaspase 9. Cytochrome c interacts with the cytochrome b-c1 complex, cytochrome oxidase complex, heme, Apaf-1, and Caspase 9 proteins. The 7H8.2C12 monoclonal antibody recognizes cytochrome-c from most species and has been shown to be useful for Western blotting.
50microl
Verified Reactivity: Human, Mouse, Rat, Hamster
Reported Reactivity: Other species
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Horse cyt c-OVA
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: Upon receipt, store undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl antibody per 5 ml antibody dilution buffer for each mini-gel. For immunocytochemistry, a concentration range of 1.0 - 3.0 µg/ml (1:150-1:500 dilution) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Additional Product Notes: The 50 µL size can be used for approximately 5 Western blots, and the 200 µL size can be used for approximately 20 Western blots.
Application References(PubMed link indicates BioLegend citation): Jemmerson R, et al. 1991. Eur. J. Immunol. 21:143. (WB) Semenkova L, et al. 2003. Eur. J. Biochem. 270:4388. (WB)
Product Citations: Kakimoto PA, et al. 2019. PLoS One. 14:e0211733. PubMed Chiang JC, et al. 2022. Antioxidants (Basel). 11:. PubMed Wu CH, et al. 2019. J Extracell Vesicles. 8:1588538. PubMed Kocabey S, et al. 2021. Nanomaterials (Basel). 11:. PubMed Asano M, et al. 2017. Sci Rep. 10.1038/s41598-017-11784-y. PubMed Duncan H 2010. Infect Immun. 78:3616. PubMed Bjedov I, et al. 2020. PLoS Genet. 16:e1009083. PubMed Liu X et al. 2018. Cell. 174(1):187-201 . PubMed Luis-Garcia ER, et al. 2021. Int J Mol Sci. 22:. PubMed
RRID: AB_2090157 (BioLegend Cat. No. 612503) AB_2292697 (BioLegend Cat. No. 612504)
Structure: Heme binding domain; 15 kD
Distribution: Mitochondrial intermembrane space
Function: Component of electron transport chain; heme group transfers electrons from cytochrome b-c1 complex to cytochrome oxidase complex. Initiates apoptosis by release to cytoplasm and binding Apaf-1 which activates procaspase 9
Interaction: Cytochrome b-c1 complex, cytochrome oxidase complex, heme, Apaf-1, Casp9
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Mitochondrial Function, Neuroscience, Neuroscience Cell Markers
Molecular Family: Mitochondrial Markers
Antigen References: 1. Liu X, et al. 1996. Cell 86:147. 2. Li P, et al. 1997. Cell 91:479. 3. Zhang Z, et al. 2003. Gene 312:61. 4. Ferguson H, et al. 2003. J. Biol. Chem. 278:45793.
Gene ID: 54205
UniProt: View information about Cytochrome c on UniProt.org
Clone: 7H8.2C12
Regulatory Status: RUO
Other Names: Cyt c
Isotype: Mouse IgG2b, κ