Biolegend, 612901, Purified anti-DNA-PKcs Phospho (Thr2609) Antibody, 25μg

DNA-protein kinase catalytic subunit (DNA-PKcs) also known as DNA-activated kinase is a nuclear 460-470 kD serine threonine kinase involved in double-stranded DNA break repair, VDJ recombination, and transcriptional modulation. DNA-PKcs must bind DNA ends to become active. DNA-PKcs is modified by phosphorylation and has been shown to interact with Ku70/Ku80, KIP, DNA-ligase IV, and XRCC4 proteins. Phosphorylated DNA-PKcs is upregulated after DNA damaage. The 10B1 monoclonal antibody recognizes phosphorylated human DNA-PKcs (Thr2609) and has been shown to be useful for immunofluorescence staining and Western blotting.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Modified peptide
Formulation: This DNA-PKcs antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store undiluted between 2°C and 8°C.
Application: ICC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 5.0 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Reindl J, et al. 2022. Sci Rep. 12:1305. PubMed Eid M, et al. 2014. J Immunol. 192:5529. PubMed
RRID: AB_315784 (BioLegend Cat. No. 612901) AB_315785 (BioLegend Cat. No. 612902)
Structure: PI3/PI4-Kinases family. Spliced products 460 kD, 470 kD
Distribution: Nuclear
Function: Serine/Threonine kinase, dsDNA break repair, VDJ recombination, transcriptional modulator
Interaction: Ku70/Ku80, KIP, DNA-ligase IV, XRCC4
Modification: Phosphorylation
Biology Area: Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication, Signal Transduction, Transcription Factors
Molecular Family: Phospho-Proteins, Protein Kinases/Phosphatase
Antigen References: 1. Hartley K, et al. 1995. Cell. 82:849. 2. Connelly M, et al. 1996. Gene. 175:271. 3. Douglas P, et al. 2002. Biochem. J. 368:243 4. Calsou P, et al. 2003. J. Mol. Biol. 326:93.
Regulation: Must bind DNA ends to become active
Gene ID: 5591
UniProt: View information about DNA-PKcs Phosphorylated on UniProt.org
Clone: 10B1
Regulatory Status: RUO
Other Names: DNA-protein kinase catalytic subunit, DNA-activated protein kinase, DNA-dependent protein kinase
Isotype: Mouse IgG1, κ