Biolegend, 613352, Purified anti-human CXCL13 Antibody, 100μg

CXCL13 was initially described as a chemokine highly expressed in the follicles of Peyer's patches, spleen, and lymph nodes and as a strong chomoattractant for B cells. CXCL13 is expressed by T follicular helper cells, dendritic cells, and stromal cells in B cell areas of secondary lymphoid tissue. CXCR5, the receptor for CXCL13, was initially cloned from Burkitt’s lymphoma cells and named BLR1. CXCR5 is highly expressed in those cells and B cells. CXCL13, CCL19, and CCL21 are involved in the formation of tertiary lymphoid tissues in chronic antigeninduced arthritis. CXCL13 takes part in the allergic airway inflammatory process. In addition, high expression of CXCL13 has been linked to lupus, Helicobacter pylori-associated gastritis, multiple sclerosis, and rheumatoid arthritis. In summary, CXCL13 is highly expressed at the sites of new lymphoid tissue formation in a variety of chronic inflammatory conditions.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human Recombinant CXCL13 Protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: Direct ELISA - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.
Additional Product Notes: This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (clone A15151A) could function as a capture antibody when paired with Biotin anti-human CXCL13 antibody (Cat. No. 612653) (clone A15151H) as the detection antibody and using Recombinant Human CXCL13 (carrier-free) protein (Cat. Nos. 574702, 574704, 574706 or 574708) as the assay standard.
RRID: AB_2941612 (BioLegend Cat. No. 613352)
Structure: Chemokine
Distribution: Follicular dendritic cells, monocytes, macrophages, and stromal cells from germinal centers. CXCL13 is derived from endothelial cells, synovial fibroblasts, and follicular dendritic cells in RA.
Function: CXCL13 chemoattracts B cells and is a key chemokine for B-cell entry into lymphoid follicles. It also takes part in the formation of ectopic lymphoid tissue in chronic inflammation. CXCL13 production by follicular Th cells is induced by TCR activation.
Interaction: B cells and follicular helper CD4 T cells.
Ligand/Receptor: CXCR5 (CD185)
Biology Area: Cell Biology, Signal Transduction
Molecular Family: Cytokines/Chemokines
Antigen References: Gunn MD, et al. 1998. Nature. 391:799-803. Legler DF, et al. 1998. J Exp Med. 187:655-60. Kim CH, et al. 2004. Blood. 104:1952-60. McDonald KG, et al. 2010. Am. J. Pathol. 176:2367. Gatto D, et al. 2011. J Immunol. 187:4621-8.
Gene ID: 10563
UniProt: View information about CXCL13 on UniProt.org
Clone: A15151A
Regulatory Status: RUO
Other Names: B cell attracting chemokine (BCA-1)
Isotype: Mouse IgG2a, κ