Biolegend, 614154, PE anti-NF-κB p65 Phospho (Ser529) Antibody, 100tests

NF-κB p65, also known as NFKB3 and RelA, is one of five members of the nuclear factor κB (NF-κB)/Rel family. Inactive NF-κB p65 is sequestered in the cytoplasm as a complex with IκB inhibitory proteins. Upon activation of the NF-κB pathway, IκB proteins are degraded, thus releasing NF-κB p65 to the nucleus. Binding of NF-κB p65 to transcriptional cofactors is induced via phosphorylation on serines 276, 529, 536, and 471. As a transcription factor, NF-κB p65 is vital to cell proliferation, immune response, survival, and apoptosis. Through regulation of chondrocyte and osteoblast differentiation and survival, NFκB p65 has a crucial role in skeletal development. Overexpressed in several cancers, NF-κB p65 functions as an oncogene and has been implicated in tumorigenesis, metastasis, tumor angiogenesis, and chemoresistance.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic peptide corresponding to human NF-κB p65 phosphorylated at serine 529
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_3097548 (BioLegend Cat. No. 614153) AB_3097548 (BioLegend Cat. No. 614154)
Structure: NF-κB p65 is a 551 amino acid protein with a predicted molecular weight of 60 kD.
Distribution: Ubiquitously expressed/ Cytoplasm and Nucleus
Function: Transcription, RAS pathway, host-virus interaction, oncogene
Interaction: NF-kappa-B, TLE5, TLE1, TP53BP2, GSK3B, MAP3K14, HDAC3, AKIP1, FOXP3
Biology Area: Cell Biology, Immuno-Oncology, Immunology, Innate Immunity, Transcription Factors
Molecular Family: Innate Immune Signaling, Phospho-Proteins, Protein Kinases/Phosphatase
Antigen References: Giridharan S and Srinivasan M. 2018. J Inflamm Res. 11:407-419. Ren C, et al. 2022. Cell Death Differ. 29:381-392. Yu S, et al. 2020. FEBS Lett. 594:13681378.
Gene ID: 5970
UniProt: View information about NF-kappaB p65 on UniProt.org
Clone: A21012B
Regulatory Status: RUO
Other Names: NF-kB p65, RelA,P65, NFKB3, Nuclear Factor NF-Kappa-B P65 subunit, Transcription Factor P65, NF-Kappa-B Transcription Factor P65, RELA Proto-Oncogene NF-KB subunit
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.