Biolegend, 614251, Purified anti-DLAT Antibody, 25μg

Dihydrolipoamide S-Acetyltransferase (DLAT) is one of three enzyme components of the pyruvate dehydrogenase complex. Specifically, DLAT catalyzes the transfer of an acetyl group to coenzyme A (CoA), which is the second step in the conversion of pyruvate into acetyl CoA during the tricarboxylic acid (TCA) cycle. Autoimmune reactivity against DLAT is the most common cause of primary biliary cholangitis, an autoimmune disease caused by autoantibodies against mitochondrial antigens. Additionally, mutations in this gene can cause pyruvate dehydrogenase E2 deficiency, resulting in primary lactic acidosis in infancy and early childhood. In line with the Warburg Hypothesis, which posits that disrupted cellular metabolism drives cancer formation, upregulation of DLAT is a characteristic of some cancers.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Purified recombinant human DLAT fragments
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This product is not recommended for use in IHC-P. This product does not recognize mouse DLAT in WB. When testing by ICC, the following fixation and permeabilization methods are suitable: ice-cold methanol, 4% PFA + 0.5% Triton-X, and 4%PFA + 100% methanol.
RRID: AB_2941616 (BioLegend Cat. No. 614251) AB_2941616 (BioLegend Cat. No. 614252)
Structure: DLAT is a 647 amino acid protein with a predicted molecular weight of 69.0 kD
Distribution: Ubiquitous/ mitochondria
Function: DLAT catalyzes the acetylation of coenzyme A
Interaction: DLAT is the E2 component of the pyruvate dehydrogenase complex along with pyruvate dehydrogenase (E1), dihydrolipoyl dehydrogenase (E3), and dihydrolipoyl dehydrogenase binding protein (E3BP)
Biology Area: Cell Biology, Mitochondrial Function
Molecular Family: Enzymes and Regulators, Mitochondrial Markers
Antigen References: Mackay IR, et al. 2000. Immunological Rev. 174:226-37. Head RA, et al. 2005. Ann Neurol. 58:234-41. Goh WQ, et al. 2015. Am J Transl Res. 7:1140-51.
Gene ID: 1737
UniProt: View information about DLAT on UniProt.org
Clone: 4A4-B6-C10
Regulatory Status: RUO
Other Names: Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial (DLAT), dihydrolipoyl transacetylase (DLTA), Pyruvate dehydrogenase complex component E2 (PDC-E2; PDCE2), Dihydrolipoamide S-Acetyltransferase
Isotype: Mouse IgG1, κ