Biolegend, 615651, Purified anti-Aldolase C Antibody, 25μg

Aldolase C is one of the three aldolase isozymes and plays a key role in ATP biosynthesis. It participates in glycolysis and gluconeogenesis by catalyzing the conversion of fructose-1-6-biphosphate to glyceraldehyde-3-phosphate, or glyceraldehyde, and di-hydroxy-acetone-phosphate. In addition, it is part of the stress-response pathway induced by hypoxia. It has been found that Aldolase C is upregulated in the brains of schizophrenia patients. Moreover, inactivation of Aldolase C by oxidation has been reported in mild cognitive impairment (MCI) and Alzheimer’s disease (AD).
25μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human Aldolase C
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P, ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 5.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 2.5 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2941622 (BioLegend Cat. No. 615651) AB_2941622 (BioLegend Cat. No. 615652)
Structure: Aldolase C is a 364 amino acid protein with an expected molecular weight of 39 kD.It is a member of the three aldolase isozymes. Aldose C shares 68% identity with Aldolase B and 78% with Aldolase A. The catalytic site is highly conserved in all three isoforms.
Distribution: Although Aldolase C is widely expressed, it is predominantly expressed in the hippocampus, Purkinje cells, neurons of the brain, and smooth muscle.
Function: Glycolytic enzyme that catalyzes the reversible aldol cleavage of fructose-1,6-biphosphate and fructose 1-phosphate to dihydroxyacetone phosphate and either glyceraldehyde-3-phosphate or glyceraldehyde, respectively.
Biology Area: Cell Biology, Neurodegeneration, Neuroscience
Molecular Family: Enzymes and Regulators
Antigen References: Chen X, et al. 2022. Nature. 605:722-727. Chang YC, et al. 2018. Trends Endocrinol Metab. 28:549-559. Juszczak GR, et al. 2017. Prog Neuropsychopharmacol Biol Psychiatry. 82:136. Lai GE, et al. 2022. Gland Surg. 11:826-836.
Gene ID: 230
UniProt: View information about Aldolase C on UniProt.org
Clone: A18070A
Regulatory Status: RUO
Other Names: Aldolase, fructose-biphosphate C, ALDC, Aldolase C, Aldolase 3, Fructoaldolase C
Isotype: Mouse IgG2b, κ