Biolegend, 615951, Purified anti-Olig2 Antibody, 25μg

Olig2 is a transcription factor that plays a crucial role in the development and maintenance of the central nervous system. This transcription factor is a master regulator for oligodendrocyte cell specification. Olig2 activates the expression of myelin-associated genes in oligodendrocyte lineage cells, which are essential for proper neuronal function and transmission of nerve impulses. In addition to its role in oligodendrocyte differentiation and maturation, Olig2 also regulates the development and maintenance of motor neurons in the spinal cord and contributes to the proliferation and differentiation of neural stem cells. Dysregulation of Olig2 expression has been associated with several neurological disorders, including multiple sclerosis and gliomas, highlighting its importance in maintaining normal brain function. Being located on chromosome 21, dysregulation of Olig2 is also associated with Down Syndrome.
25μg
Verified Reactivity: Human, Rat
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of the N-terminus of mouse Olig2
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested ICC, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 10.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is 0.25 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.
Additional Product Notes: For use in intracellular flow cytometry (ICFC), it is recommended to fix/permeabilize with either True-Phos™ Perm Buffer (Cat. No. 425401) or with Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803). Use of TrueNuclear™ Transcription Factor Buffer Set (Cat. No. 424401) is not recommended.
RRID: AB_2941623 (BioLegend Cat. No. 615951) AB_2941623 (BioLegend Cat. No. 615952)
Structure: Olig2 is a 323 amino acid protein with a predicted molecular weight of 32.38 kD.
Distribution: Nucleus
Function: Transcription factor
Cell Type: Neural Stem Cells, Oligodendrocytes
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers, Transcription Factors
Antigen References: Zhang K, et al. 2022. Nat Commun. 13:1423. Xu R, et al. 2019. Cell Stem Cell. 24:908-926. Sock E, Wegner M. 2021. Dev Neurobiol. 81:892-901. Szu J, et al. 2021. Front Neurosci. 15:659601.
Gene ID: 10215
UniProt: View information about Olig2 on UniProt.org
Clone: W21023A
Regulatory Status: RUO
Other Names: Oligodendrocyte transcription factor 2 (Olig2), Class B basic helix-loop-helix protein 1 (bHLHb1), Class E basic helix-loop-helix protein 19 (bHLHe19), Protein kinase C-binding protein 2 (PRKCBP2), RACK17, OLIGO2
Isotype: Rat IgG2b, κ