Biolegend, 616001, Purified anti-LEF1 Antibody, 25μg

Lymphoid Enhancing Factor 1 (LEF1) is a transcription factor belonging to the TCF/LEF family. LEF1 was first identified as a protein binding to TCR-α enhancer and upregulates expression of T-cell antigen receptor α chain. LEF1 is involved in Wnt signaling pathways as a ubiquitous regulator. During Wnt signaling, translocation of β-catenin from the cytosol to the nucleus results in elevation of transcriptional activity of LEF1, which subsequently regulates multiple downstream genes.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant human LEF1 protein corresponding to amino acid residues 116-283
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedICC, IP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 µg per ml. For immunocytochemistry, a concentration of 5.0 μg/ml is recommended. For immunoprecipitation, the suggested use of this reagent is 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: W17155A recognizes multiple isoforms of LEF1.When tested for ICC, W17155A failed to stain PFA-fixed Jurkat cells permeabilized with methanol. We recommend Triton X-100 permeabilization.
RRID: AB_2814460 (BioLegend Cat. No. 616001) AB_2814461 (BioLegend Cat. No. 616002)
Structure: LEF1 is a 399 amino acid protein with a predicted molecular weight of 44 kD. Seven total isoforms have been reported, ranging in size from 23 to 44 kD.
Distribution: Lymphoid tissue enriched/Nuclear localization
Function: Canonical Wnt signaling
Biology Area: Angiogenesis, Cell Biology, Immunology, Signal Transduction, Transcription Factors
Molecular Family: Nuclear Markers, TCRs
Antigen References: 1. Mallory MJ, et al. 2011. Mol. Cell. Biol. 31:2184. 2. Waterman ML, et al. 1991. Genes Dev. 5:656. 3. Hovanes K, et al. 2001. Nat Genet. 28:53. 4. Bruhn L, et al. 1997. Genes Dev. 11:640. 5. Nguyen A, et al. 2005. Int. J. Oncol. 27:949. 6. Petropoulos K, et al. 2008. J. Exp. Med. 205:515.
Gene ID: 51176
UniProt: View information about LEF1 on UniProt.org
Clone: W17155A
Regulatory Status: RUO
Other Names: Lymphoid enhancer-binding factor 1, TCF1α, TCF10
Isotype: Rat IgG2b, κ