Biolegend, 616452, Purified anti-CD340 (ErbB2/HER-2) Phospho (Tyr1248) Antibody, 200microl

CD340, also commonly known as HER2 or ErbB2, is a member of the epidermal growth factor receptor family of cell membrane tyrosine kinases containing a single transmembrane domain. CD340 contains three furin repeats and two cheY homologous receiver domains in the extracellular region. Although CD340 itself contains no ligand binding domain, it interacts with other EGF receptor family members to form a heterodimer, stabilize ligand binding, and enhance kinase-mediated downstream signaling. CD340 has been shown to be involved in embryonic development and cancer progression; this protein is amplified in adenocarcinoma of the lung, some breast cancers, glioblastoma, gastric cancer, and ovarian carcinoma, where it functions as an oncogene. Overexpression of CD340 in breast cancers has been shown to confer Taxol resistance, and CD340 is a therapeutic target in a number of human cancers. CD340 activation is accompanied by autophosphorylation at multiple tyrosine residues including Tyr1248.
200microl
Verified Reactivity: Human
Antibody Type: Polyclonal
Host Species: Rabbit
Immunogen: Synthetic peptide of human CD340 phosphorylated at Tyr1248
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_3068238 (BioLegend Cat. No. 616451)
Structure: CD340 is a 1,259 amino acid protein with a predicted molecular weight of 137.9 kD.
Distribution: Plasma membrane
Function: Proto-oncogene
Interaction: CD340 interacts with a large number of proteins, including SHC1, EGF receptor, neuroregulin 1, c-Src, integrin β4, Grb2, Grb7, SOS1, caveolin 1, JAK2, PAK1, FAK, and β-catenin, among others.
Ligand/Receptor: Unlike other members of the EGFR family, CD340 does not contain a ligand binding domain
Cell Type: Leukemia
Biology Area: Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules, Phospho-Proteins
Antigen References: Akiyama T, et al. 1986. Science. 232:1644-6. Bargmann CI, et al. 1986. Nature. 319:226-30. Pegram MD, et al. 1997. Oncogene. 15:537-47. Slamon DJ, et al. 1989. Science. 244:707-12. Leone F, et al. 2003. J Leukoc Biol. 74:593-601.
Gene ID: 2064
UniProt: View information about ErbB2 Phospho Tyr1248 on UniProt.org
Clone: Poly22A04
Regulatory Status: RUO
Other Names: Receptor tyrosine-protein kinase erbB-2 (ERBB2), human epidermal growth factor receptor 2 (HER2), metastatic lymph node gene 19 protein (MLN19), Proto-oncogene Neu, p185(erbB2), cluster of differentiation 340 (CD340), NGL, herstatin
Isotype: Rabbit Polyclonal IgG