Biolegend, 616602, Purified anti-TGM2 Antibody, 100μg

Transglutaminase 2 (TGM2) is a multifunctional enzyme required for diverse cellular processes, including cell death, differentiaton, apoptosis, and cell migration. TGM2 catalyzes the crosslinking through calcium-activated transamidation of available glutamine and lysine residues on substrate proteins. TGM2 also has GTPase and ATPase activity; transamidation activity is allosterically inhibited by guanine nucleotide binding. It is essential for cancer stem cell survival and is a chemotherapy resistance factor.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial human recombinant human TGM2 protein corresponding to amino acids 1-250.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone was tested for ICC using PFA-fixed A431 cells permeabilized with Triton X-100 or methanol. Staining in the incorrect subcellular compartment was observed using each method; we therefore do not recommend this clone for ICC. This clone failed to immunoprecipitate TGM2 during PD testing.
RRID: AB_2810665 (BioLegend Cat. No. 616601) AB_2810666 (BioLegend Cat. No. 616602)
Structure: TGM2 is a 687 amino acid protein with a predicted molecular weight of 77 kD.
Distribution: Tissue enriched/cytosol localization
Function: Post translational modification, GTPase activity.
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Structure
Molecular Family: Enzymes and Regulators
Gene ID: 7052
UniProt: View information about TGM2 on UniProt.org
Clone: W17122A
Regulatory Status: RUO
Other Names: C Polypeptide, Protein-Glutamine-Gamma-Glutamyltransferase, Protein-Glutamine Gamma-Glutamyltransferase 2, Transglutaminase C, Transglutaminase H, Tissue Transglutaminase, Transglutaminase 2
Isotype: Rat IgG2a, κ