Biolegend, 616701, Purified anti-NF-κB p50 Antibody, 25μg

NF-κB p50 (nuclear factor kappa light chain enhancer in B cells p50, NF-κB1) is a member of the Rel/dorsal family. NF-κB is a transcription factor, originally identified as an activator of kappa light chain in B cells. NF-κB p50, together with another NF-κB subunit p65, binds to IκB inhibitor in cytoplasm as an inactive form. Phosphorylation of IκB releases NF-κB p50 and p65. Active form of NF-κB p50 and p65 then translocate to the nucleus and regulate target gene expression. Bacterial products and stimulation of a variety of receptors lead to NF-κB signal pathway activation. This 4D1 monoclonal antibody recognizes p105 (precursor of p50) and p50 (active form). 4D1 has been validated for Western blotting.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: recombinant full-length human NF-κB p50
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store between 2°C and 8°C.
Application: WB - Quality tested ICC - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.025 - 0.5 µg per ml. For immunocytochemistry, a concentration range of 2.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: immunofluorescence1 on the Amnis ImageStream 100 Imaging Flow Cytometer. This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).
Application References(PubMed link indicates BioLegend citation): Huang F, et al. 2007. J. Immunol. 179:6504. (IF) PubMed Cao YA, et al. 2008. J. Biol. Chem. 283:15309. PubMed Lin D, et al. 2012. J Biol Chem. 287:4299. PubMed.
Product Citations: Lin D, et al. 2012. J Biol Chem. 287:4299. PubMed Herati RS, et al. 2021. Cell Reports Medicine. 2(5):100262. PubMed Kao C, et al. 2008. J Biol Chem. 283:15309. PubMed Boudesco C, et al. 2018. Blood. 132:510. PubMed Huang F, et al. 2007. J Immunol. 179:6504. PubMed
RRID: AB_315855 (BioLegend Cat. No. 616701) AB_315856 (BioLegend Cat. No. 616702)
Structure: Member of the Rel/dorsal family. NF-κB p50 is an active form, derived from NF-κB p105, a precursor form.
Distribution: Ubiquitously expressed
Function: A transcription factor, originally identified as an activator of kappa light chain in B cells
Interaction: Interacts with NF-κB p65 and IκB
Biology Area: Cell Biology, Immunology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Baldwin AS, et al. 1996. Annu. Rev. Immunol. 14:649 (review). 2. Chen F, et al. 1999. Clin. Chem. 45:7 (review).
Regulation: NF-κB p50, together with another NF-κB subunit p65, binds to IκB inhibitor in cytoplasm as an inactive form. Phosphorylation of IκB releases NF-κB p50 and p65. Active form of NF-κB p50 and p65 then translocate to the nucleus and regulate target gene expression. Bacterial products and stimulation of a variety of receptors lead to NF-κB signal pathway activation.
Gene ID: 4790
UniProt: View information about NF-kappaB p50 on UniProt.org
Clone: 4D1
Regulatory Status: RUO
Other Names: NF-κB p50, nuclear factor kappa light chain enhancer in B cells p50, NF-κB1
Isotype: Mouse IgG1, κ