Biolegend, 617601, Purified anti-PDPK1 Antibody, 25μg

3-Phosphoinositide-Dependent Protein Kinase 1 (PDPK1) is serine/threonine kinase that plays a central role in diverse cellular pathways. It functions by phosphorylating and activating members of the AGC family of serine kinases, including Akt1, Protein Kinases A and C, and p70 ribosomal protein S6 kinase. Loss or reduction of PDPK1 in the thymus results in defective T-cell differentiation and proliferative expansion.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant human PDPK1 protein corresponding to amino acid residues 411-556
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone is predicted to recognize rat PDPK1 due to high sequence conservation between the immunizing protein and the rat homolog.This clone is predicted to recognize PDPK1 isoforms 1-4 due to the presence of the immunizing sequence in each isoform.This clone failed ICC and IP testing.
RRID: AB_2810673 (BioLegend Cat. No. 617601) AB_2810674 (BioLegend Cat. No. 617602)
Structure: PDPK1 is a 556 amino acid protein with a predicted molecular weight of 63 kDa. Four PDPK1 isoforms exist ranging in size from 48-63 kD.
Distribution: Peripheral plasma membrane protein/ubiquitously expressed
Function: Regulator of insulin, PKC, Akt1, and SMAD signal tranduction
Biology Area: Cell Biology
Molecular Family: Protein Kinases/Phosphatase
Antigen References: Le Good JA, et al. 1998. Science. 281:2042. Dong LQ, et al. 2000. Proc. Natl. Acad. Sci. 97:5089. Hinton HJ, et al. 2004. Nat. Immunol. 5:539.
Gene ID: 5170
UniProt: View information about PDPK1 on UniProt.org
Clone: W17215A
Regulatory Status: RUO
Other Names: 3-Phosphoinositide-Dependent Protein Kinase 1, PDK1
Isotype: Rat IgG2b, κ