Biolegend, 617904, Ultra-LEAF™ Purified anti-human β-NGF Antibody, 1mg

Neurotrophins are growth factors that modulate growth, differentiation, and survival of neurons. These structurally related proteins include nerve growth factor (NGF/β-NGF), brain-derived neurogenic factor (BDNF), neurotrophin-3, and neurotrophin-4/5. They are highly homologous and share a similar structure of a tertiary folded, cysteine rich "knot". Neurotrophins are critical to the development of the central and peripheral nervous systems, and they play important roles in injury-induced neuronal regeneration. β-NGF is a member of the neurotrophin family which controls growth, survival, and maturation of neurons. In peripheral and central neurons, neurotrophins are important regulators for survival, differentiation, and maintenance of nerve cells. They are detectable throughout embryogenesis and into the adult stage in the central and peripheral nervous systems. In animal models, β-NGF prevents neuronal degeneration and has become a therapeutical target in neuron regeneration studies. β-NGF is also critical for the survival and maintenance of sympathetic and sensory neurons. It is the main neurotrophin that maintains homeostasis of neurogenesis. It prevents neurons from undergoing apoptosis and promotes maturation while supporting axonal branching. Two receptors are known to interact with NGF, p75NTR and TrkA. The NGF-TrkA signaling pathway induces two pathways through activation of PI3K-Akt or Ras-MAPK. Either one of the pathways leads to CREB phosphorylation and increases expression of anti-apoptosis genes to keep neurons alive. Animal models of neurodegenerative diseases suggest that NGF can promote myelin repair of neurons in the peripheral nervous system. Clinical studies indicate that dysregulation of NGF signaling is related to Alzheimer's disease.
1mg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant Human β-NGF
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: Neut - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by neutralization of the proliferation induced by recombinant human β-NGF (0.08 µg/mL) on TF-1 human erythroleukemia cells. ND50 range: 0.1 - 0.8 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Wu BW, et al. 2022. Viruses. 14:. PubMed
RRID: AB_2814462 (BioLegend Cat. No. 617903) AB_2814463 (BioLegend Cat. No. 617904)
Structure: Dimer
Distribution: Hypothalamus, pituitary, thyroid gland, testes, epididymis, vascular smooth muscle cells, fibroblasts, myofibroblasts, mast cells, eosinophils.
Function: β-NGF is important for maintaining neuron homeostasis.
Interaction: Neurons, astrocytes, eosinophils, B, T, and mast cells.
Ligand/Receptor: Low-affinity NGF receptor (p75NTR); high-affinity NGF receptor (TrkA).
Cell Type: B cells, Eosinophils, Fibroblasts, Mast cells, Mature Neurons, Neural Stem Cells, Neurons, T cells
Biology Area: Cell Biology, Cell Proliferation and Viability, Neurodegeneration, Neuroscience, Synaptic Biology
Molecular Family: Cytokines/Chemokines, Growth Factors
Antigen References: Ullrich A, et al. 1983. Nature. 303:821. Klein R, et al. 1991. Cell. 65:189. Welker P, et al. 2000. Immunology 99:418. Fahnestock M, et al. 2004. Prog. Brain Res. 146:101. Bruno MA. Cuello AC. 2006. Proc. Natl. Acad. Sci. USA 103:6735. Aloe L, et al. 2015. Curr. Neuropharmacol. 13:294.
Gene ID: 4803
UniProt: View information about beta-NGF on UniProt.org
Clone: A16145B
Regulatory Status: RUO
Other Names: Beta-Nerve growth factor, Nerve Growth Factor, NGFB
Isotype: Mouse IgG2a, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.