Biolegend, 618551, Purified anti-GSK3β Phospho (Ser9) Antibody, 25μg

Glycogen synthase kinase 3 beta or GSK3β, is a 420 aa length constitutively active serine-threonine kinase that phosphorylates more than 100 target proteins. GSK3β is involved in several biological activities including glycogen metabolism, cell signaling, cellular transport, cell proliferation, apoptosis, and others. The activity of GSK3β is regulated by phosphorylation at different residues. Phosphorylation at tyrosine 216 enhances GSK3β kinase activity, while phosphorylation at serine 9 decreases kinase activity. In addition, GSK3β activity can also be regulated by cellular localization and/or formation of protein complexes. GSK3β has been associated with several diseases including type 2 diabetes, Alzheimer’s disease, inflammation, cancer, addiction, and bipolar disorders.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic peptide from human GSK3β phosphorylated at Serine 9
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: ICFC: Cells were fixed and permeabilized using the True-Phos™ Buffer system
RRID: AB_3068059 (BioLegend Cat. No. 618551) AB_3068059 (BioLegend Cat. No. 618552)
Structure: GSK3β is a 420 a.a protein with a predicted molecular weight approximately 47 kD. GSK3β has a bi-lobar architecture, with N-terminal and C- terminal domains. The N-terminal is responsible for ATP binding, while the C-terminal carries the activation loop that mediates the kinase activity.
Distribution: GSK3β can be found mainly in the cytoplasm, however, it could also be present in mitochondria and nucleus.
Function: It is a serine/threonine Kinase, its activity can be enhanced by phosphorylation on its tyrosine 216, or reduced by phosphorylation on its serine 9.
Interaction: Glycogen synthase, Beta-catenin/Wnt, Insulin receptor, IRS1, MAPT/Tau proteins, NF-κB, MUC1, MACF1, E2F1, mTORC2, JUN, NFATC
Biology Area: Cancer Biomarkers, Cell Biology, Immuno-Oncology
Molecular Family: Phospho-Proteins
Antigen References: Edderkaoui M, et al. 2018. Gastroenterology. 155:1985-1998.e5. Xing B, et al. 2018. Glia. 66:1999-2012. Ryu HY, et al. 2021. Exp Mol Med. 53:369-383. Wu H, et al. 2019. Autophagy. 15:1506-1522.
Gene ID: 2932
UniProt: View information about GSK3b Phospho (Ser9) on UniProt.org
Clone: A21018C
Regulatory Status: RUO
Other Names: Glycogen synthase kinase-3 beta; GSK3B, GSK-3B, GSK-3beta
Isotype: Mouse IgG1, κ