Biolegend, 618754, Ultra-LEAF™ Purified anti-mouse CD96 (TACTILE) Antibody, 1mg

CD96, also known as TACTILE (T cell-activated increased late expression), is a type I transmembrane glycoprotein, belonging to the Ig superfamily. It is expressed on NK cells and T cells (upregulated after activation), but not on normal B cells and myeloid cells. CD96 is also found on some acute myeloid leukemia (AML) cells, and T cell acute lymphoblastic leukemia (T-ALL), as a leukemia stem cell-specific marker. A soluble form of CD96 has been found in serum. Human CD96, but not mouse CD96, is expressed as two isoforms due to alternative splicing. The main ligand of CD96 is CD155 (PVR), along with CD226 (DNAM-1) and TIGIT, and the binding is species-specific. Mouse CD96 has been reported to interact with nectin-1 (CD111), but human CD96 does not. CD96 may play an inhibitory role in immune responses. In CD96 deficient mice, NK cells produce more IFN-γ upon LPS treatment. In addition, CD96 negatively regulates NK cell function in mice, and blockade of CD96 with antibodies promotes anti-tumor responses and anti-metastatic activity in combination with anti-CTLA-4 or anti-PD-1. CD96 is considered to mediate activated T cell and NK cell adhesion to CD155-expressing target cells. CD96 also counterbalances CD226, which activates NK cell-mediated cytotoxicity, via competing CD155 binding.
1mg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant mouse CD96 (TACTILE)
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: Block - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by blocking the binding of 0.5 µg/mL recombinant mouse CD96 (TACTILE) (Cat. No. 788804) to immobilized 2 µg/mL recombinant mouse CD155-Fc Chimera (Cat. No. 786606). ND50 = 0.1 - 1 µg/mL.
RRID: AB_3068077 (BioLegend Cat. No. 618753) AB_3068077 (BioLegend Cat. No. 618754)
Structure: Ig superfamily
Distribution: Positive on most peripheral T cells, NK cells and upregulated when activated
Function: CD96 is involved in NK cell adhesion and cytotoxicity via CD155
Ligand/Receptor: CD155, nectin 1
Cell Type: NK cells, NKT cells, T cells
Biology Area: Adaptive Immunity, Immunology
Molecular Family: Soluble Receptors
Antigen References: Wang PL, et al. 1992. J Immunol. 148:2600-8. Meyer D, et al. 2009. J Biol Chem. 284:2235-44. Hosen N, et al. 2007. Proc Natl Acad Sci USA. 104:11008-13. Wen D, et al. 2015. Mol Clin Oncol. 3:833-838. Blake SJ, et al. 2016. Clin Cancer Res. 22:5183-5188. Chan CJ, et al. 2014. Nat Immunol. 15:431-8. Sanchez-Correa B, et al. 2019. Cancer. 11:877. Xu Z, Jin B. 2010. Cell Mol Immunol. 7:11-9. Georgiev H, et al. 2018. Front Immunol. 9:1072. Fuchs A, et al. 2004. J Immunol. 172:3994-8. Seth S, et al. 2007. Biochem Biophys Res Commun. 364:959-65.
Gene ID: 84544
UniProt: View information about CD96 on UniProt.org
Clone: W20256A
Regulatory Status: RUO
Other Names: T-cell surface protein tactile, TACTILE, Cell surface antigen CD96, T cell-activated increased late expression protein
Isotype: Rat IgG2b, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.