Biolegend, 618851, Purified anti-SLP76 Phospho (Tyr128) Antibody, 25μg

SLP76 is a cytosolic adaptor protein that functions as a substrate in the T cell receptor (TCR) activation pathway. Upon TCR engagement, SLP76 is phosphorylated on multiple residues by ZAP70, resulting in the protein being translocated to the plasma membrane and facilitating the assembly of a multi-subunit complex comprised of Vav1, Nck, ITK, and PLCγ1 that activates multiple signaling pathways. Mutation and inactivation of the phosphorylation sites results in abrogation of SLP76 activity. Tyr128 is one of three residues on the N-terminus of SLP76 which becomes phosphorylated upon TCR ligation, which initiates immune cell synapse formation. SLP76 also plays an essential role in normal T cell development, and mast cell and platelet function.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic human SLP76 peptide phosphorylated at Tyr128
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ICC - Quality tested ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 2.5 - 5.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone is not suitable for Western Blot application. Although the antibody does detect phospho-SLP76, there is a prominent non-specific band around 35 kDa. For immunocytochemistry, successful staining was accomplished using fixation with 4% PFA for 10 minutes followed by permeabilization with either 100% ice-cold methanol or 0.5% Triton-X for 10 minutes, or with fixation/permeabilization with 100% ice-cold methanol for 10 minutes. For flow cytometry, we recommend treatment with Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803).
RRID: AB_3083403 (BioLegend Cat. No. 618851) AB_3083403 (BioLegend Cat. No. 618852)
Structure: SLP76 is a 533 amino acid protein with a predicted molecular weight of ~60 kD.
Distribution: Hematopoietic cells/cytosol
Function: Adaptor protein/TCR Signaling
Interaction: ZAP70, ITK, LAT, GRB2, PLCγ1, NCK, VAV, ITK, LCK, 14-3-3ε
Cell Type: B cells, Monocytes, NK cells, T cells
Biology Area: Cell Biology, Immunology, Signal Transduction
Molecular Family: Phospho-Proteins, Protein Kinases/Phosphatase
Antigen References: Sauer K, et al. 2001. J Biol Chem. 276:45207-16. Koretzky GA, et al. 2006. Nat Rev Immunol. 6:67-78. Di Bartolo V, et al. 2007. J Exp Med. 204:681-91. Lasserre, et al. 2011. J Cell Biol. 195:839-53. Wardenberg, et al. 1996. J Biol Chem. 271:33.
Gene ID: 3937
UniProt: View information about SLP76 Phospho (Tyr128) on UniProt.org
Clone: A20007B
Regulatory Status: RUO
Other Names: Lymphocyte cytosolic protein 2 (LCP2), SH2 domain-containing leukocyte protein of 76 kDa (SLP76), IMD81
Isotype: Mouse IgG1, κ