Biolegend, 619302, Purified anti-TIF1β (KAP-1, TRIM28) Antibody, 100μg

TIFβ (transcription intermediary factor 1-beta) is an 88 kD member of the tripartite motif family. This protein contains three zinc binding domains, a RING domain, a B-box type 1 and type 2 domain, and a coiled-coil region. TIFβ is found in the nucleus and associates with specific chromatin regions. This protein forms a complex with KRAB-domain transcription factors and recruits SETDB1 to histone 3 to increase KRAB-mediated transcriptional repression. TIF1β has been reported to interact with SETDB1 and CBX3 proteins. Studies using knockout mice reveal the important function of TIF1β in regulating genomic imprinting, T cell activation, and T cell tolerance.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store between 2°C and 8°C.
Application: WB - Quality tested ICC - Verified ChIP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.025 - 0.1 µg per ml. For immunocytochemistry, a concentration range of 2.5 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: immunofluorescence1, Western blotting1 and chromatin immunoprecipitation1. This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing). This clone may produce increased background by western blot when used at concentrations exceeding 0.1 µg/mL.
Application References(PubMed link indicates BioLegend citation): Chang CW, et al. 2008 BMC Mol. Biol. 9:61. (WB, IF, ChIP)
Product Citations: Yang DM, et al. 2024. Nat Commun. 780:15. PubMed Ouyang C, et al. 2022. Int J Biol Sci. 18:2655. PubMed
RRID: AB_2209724 (BioLegend Cat. No. 619301) AB_2209723 (BioLegend Cat. No. 619302)
Structure: Tripartite motif family, contains three zinc-binding domains, a RING, a B-box type 1 and 2 domain, and a coiled-coil region. Predicted molecular weight approximately 110 kD
Distribution: Nuclear, associates with specific chromatin regions
Function: Forms a complex with KRAB-domain transcription factor and recruits SETDB1 to histone 3 to increase KRAB-mediated transcriptional repression
Biology Area: Cell Biology, Immunology, Transcription Factors
Molecular Family: Protein Kinases/Phosphatase
Antigen References: 1. Ryan RF, et al. 1999. Mol. Cell. Biol. 19:4366. 2. Schultz DC, et al. 2002. Genes Dev. 16:919. 3. Moosmann PR, et al. 1996. Nucleic Acids Res. 24:4859. 4. Friedman JR, et al. 1996. Genes Dev. 10:2067. 5. Messerschmidt DM, et al. 2012. Science 335:1499. 6. Chikuma S, et al. 2012. Nat. Immunol. 13:596.
Gene ID: 10155
UniProt: View information about TIF1beta on UniProt.org
Clone: 20A1
Regulatory Status: RUO
Other Names: Transcription intermediary factor 1-beta, KRAB-associated protein, TRIM28, KAP-1
Isotype: Mouse IgG1, κ