Biolegend, 619452, Purified anti-CD340 (erbB2/HER-2) Phospho (Tyr1248) Antibody, 100μg

CD340, also commonly known as ErbB2 or HER2, is a member of the epidermal growth factor receptor family of cell membrane tyrosine kinases containing a single transmembrane domain. CD340 contains three furin repeats and two cheY homologous receiver domains in the extracellular region. Although CD340 itself contains no ligand binding domain, it interacts with other EGF receptor family members to form a heterodimer, stabilize ligand binding, and enhance kinase-mediated downstream signaling. CD340 is involved in embryonic development and cancer progression; this protein is amplified in adenocarcinoma of the lung, some breast cancers, glioblastoma, gastric cancer, and ovarian carcinoma, where it functions as an oncogene. Overexpression of CD340 in breast cancers has been shown to confer Taxol resistance, and CD340 is a therapeutic target in a number of human cancers. CD340 activation is accompanied by autophosphorylation at multiple tyrosine residues including Tyr1248.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic human CD340 peptide phosphorylated at Tyr1248
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: A22018C is not suitable for ICC or ICFC
RRID: AB_3068143 (BioLegend Cat. No. 619451) AB_3068143 (BioLegend Cat. No. 619452)
Structure: CD340 is a 1,255 amino acid protein with a predicted molecular weight of 137.9 kD.
Distribution: Plasma membrane, cytoplasm, nucleus
Function: Proto-oncogene, protein tyrosine kinase receptor
Interaction: CD340 interacts with a large number of proteins, including SHC1, EGF receptor, neuroregulin 1, c-Src, integrin β4, Grb2, Grb7, SOS1, caveolin 1, JAK2, PAK1, FAK, and β-catenin, among others.
Cell Type: Leukemia
Biology Area: Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules, Phospho-Proteins
Antigen References: Akiyama T, et al. 1986. Science. 232:1644-6. Bargmann CI, et al. 1986. Nature. 319:226-30. Pegram MD, et al. 1997. Oncogene. 15:537-47. Slamon DJ, et al. 1989. Science. 244:707-12. Leone F, et al. 2003. J Leukoc Biol. 74:593-601.
Gene ID: 2064
UniProt: View information about CD340 on UniProt.org
Clone: A22018C
Regulatory Status: RUO
Other Names: Receptor tyrosine-protein kinase erbB-2 (ERBB2), human epidermal growth factor receptor 2 (HER2), metastatic lymph node gene 19 protein (MLN19), Proto-oncogene Neu, p185erbB2, cluster of differentiation 340 (CD340), NGL, VSCN2, TKR1
Isotype: Mouse IgG1, κ