Biolegend, 619602, Purified anti-4EBP1 Phospho (Thr70) Antibody, 100μg

In eukaryotes, the mTOR kinase signaling pathway links cell growth and proliferation with nutrient availability. Under nutrient-deplete conditions, eukaryotic translation initiations factor 4E-binding protein 1 (4EBP1) binds the general translation factor eIF4E, resulting in an attenuation of cap-dependent translation. Under nutrient-replete conditions, mTORC1 and mTORC2 complexes phosphorylate 4EBP1 at Threonine 70 and disrupt the formation of the 4EBP1-eIF4E complex, thereby alleviating the repressive function of 4EBP1.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic peptide corresponding to human 4EBP1 phosphorylated at Threonine 70
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone has not been tested for ICC and IP.
RRID: AB_2814468 (BioLegend Cat. No. 619601) AB_2814469 (BioLegend Cat. No. 619602)
Structure: 4EBP1 is a 118 amino acid protein with a predicted molecular weight of 13 kD.
Distribution: Cytosol and nucleus/ubiquitously expressed
Function: Regulation of global translation
Biology Area: Cell Biology, Cell Proliferation and Viability, Protein Synthesis, Signal Transduction
Molecular Family: Phospho-Proteins
Antigen References: Brunn GJ, et al. 1997. Nature. 277:99. Hara K, et al. 2002. Cell. 110:177. Ekim B, et al. 2011. Mol. Cell Biol. 31:2787.
Gene ID: 1978
UniProt: View information about 4EBP1 on UniProt.org
Clone: A18010A
Regulatory Status: RUO
Other Names: Eukaryotic Translation Initiation Factor 4E Binding Protein 1, Phosphorylated Heat- And Acid-Stable Protein Regulated By Insulin 1, PHAS-I
Isotype: Mouse IgG1, κ