Biolegend, 620001, Purified anti-ATG17 Antibody, 25μg

RB1-inducible coiled-coil 1 (RB1CC1), also known as ATG17 or FIP200, was originally identified as a potent regulator of RB1 gene. RB1CC1 is a component of the ULK1-ATG13-RB1CC1 complex, which is required for the early events of autophagy and is inhibited by mTOR. RB1CC1 has been suggested to participate in the NF-kβ, mTOR, and FAK-Erk1/2 signaling pathways and regulates cell proliferation, apoptosis, and migration. RB1CC1 enhances transcription of RB1, p16, and p21 when forms a complex with p53, PIASy, and hSNF5. RB1CC1 plays an important role in tumor suppression by globally activation of the RB1 pathway. Aberrant expression or genetic alterations of RB1CC1 has been associates with various disease, including cancer, neuronal degeneration, skin inflammation, and anemia.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant human RB1CC1 (1345-1594 a.a.) expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 2.0 µg per ml (1:250-1:2500 dilution). It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone does not work for IF microscopy application (in-house tested).
RRID: AB_2716038 (BioLegend Cat. No. 620001) AB_2716039 (BioLegend Cat. No. 620002)
Structure: 1594 amino acids with a predicted molecular weight of 183 kD. Contains two C-terminal coiled coil domains.
Distribution: Nucleus, Cytoplasm.
Function: RB1CC1 is a mammalian homolog of yeast ATG17 and is involved in the regulation of cell growth, proliferation, apoptosis, migration, and autophagy.
Interaction: RB1CC1 interacts with ULK1, ATG13, FAK1, PYK2, p53, RFWD2, GABARAPL1, GABARAP, ATG16L1, RNF111, SKI, and SMAD7.
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Neuroscience, Neuroscience Cell Markers, Transcription Factors
Molecular Family: Autophagosome Markers, Nuclear Markers
Antigen References: 1. Zhang LY, et al. 2016. Carcinogenesis. 37:320. 2. Yao J, et al. 2015. Autophagy. 11:939. 3. Suraneni MV, et al. 2014. Cell Cycle. 13:1798. 4. Morselli E, et al. 2011. Cell Cycle. 10:2763. 5. Ikebuchi K, et al. 2009. Int. J. Cancer. 125:861. 6. Chano T, et al. 2002. Nat Genet. 31:285.
Gene ID: 9821
UniProt: View information about ATG17 on UniProt.org
Clone: W16188A
Regulatory Status: RUO
Other Names: RB1-inducible coiled-coil protein 1, FAK family kinase-interacting protein of 200 Da,FIP200, ATG17, KIAA0203
Isotype: Rat IgG2a, κ