Biolegend, 620302, Purified anti-SHP-1 Antibody, 100μg

SHP-1, also referred to as PTPN6 is an inhibitory protein phosphatase widely expressed in hematopoietic cell lineages that regulates cell cycle, proliferation, and inflammatory pathways. SHP-1 dephosphorylates activating signaling molecules in receptor tyrosine kinase pathways; in T cells it functions as a negative regulator of T cell activation and proliferation. SHP-1 is constitutively bound to the ITIM-containing adaptor molecules such as the inhibitory receptor LAIR-1; activation of SHP-1 results in either direct dephosphorylation of the T cell receptor or downstream effector molecules such as Zap70 and Lck. Reduced expression of SHP1 is found in leukemias, lymphomas, and colorectal cancers due to promoter hypermethylation of the PTPN6 gene.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant protein corresponding to amino acid residues 1-213 of human SHP-1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, IP, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For immunoprecipitation, the suggested use of this reagent is 2.5 µg per mL. For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: W17240D was tested for ICC using PFA-fixed Jurkat cells permeabilized with either Triton X-100 or methanol. While both permeabilization methods facilitated SHP-1 staining, methanol resulted in considerably stronger staining.
Product Citations: Tathe P, et al. 2022. EMBO J. 41:e109720. PubMed Lam W, et al. 2023. Front Pharmacol. 13:1095186. PubMed Huang CY, et al. 2022. Int J Mol Sci. 23:. PubMed
RRID: AB_2810679 (BioLegend Cat. No. 620301) AB_2810680 (BioLegend Cat. No. 620302)
Structure: SHP-1 is a 595 amino acid protein with a predicted molecular weight of 68 kD.
Distribution: Cytoplasm and nucleus/hematopoietic cells
Cell Type: T cells, Thymocytes
Biology Area: Cell Biology, Cell Proliferation and Viability, Immuno-Oncology, Immunology
Molecular Family: Protein Kinases/Phosphatase
Antigen References: Paulson RF, et al. 1996. Nat Genet. 13:309. Khoury JD, et al. 2004. Blood. 104:1580. Paster W, et al. 2015. EMBO J. 34:393.
Gene ID: 5777
UniProt: View information about SHP-1 on UniProt.org
Clone: W17240D
Regulatory Status: RUO
Other Names: Protein Tyrosine Phosphatase, Non-Receptor Type 6, PTPN6, Hematopoietic Cell Protein-Tyrosine Phosphatase, Protein-Tyrosine Phosphatase SHP-1, Protein-Tyrosine Phosphatase 1C
Isotype: Rat IgG2b, κ