Biolegend, 620602, Purified anti-TPX2 Antibody, 100μg

TPX2 protein, also known as targeting protein for Xklp2, is strictly associated with the spindle pole and mitotic spindle during mitosis. In the G2/S position of the cell cycle, TPX2 is diffusely distributed throughout the nucleus. TPX2 has been shown to be highly expressed in lung carcinoma cell lines, but not in normal lung tissues. TPX2 is thought to be required for the Ran-GTP dependent assembly of microtubules around chromosomes required to generate stable bipolar spindles with overlapping anti-parallel microtubule arrays and may also be involved in targeting Aurora-A kinase to the mitotic spindle. TPX2 has been shown to interact with a large number of proteins including serine/threonine protein kinase 6, ribosomal protein 6, Bop-1, α-tubulin, and nucleolin among others. TPX2 can be modified by phosphorylation on serine 738.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Synthetic peptide corresponding to amino acid residues 413-429 of human TPX2 protein.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, IP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 µg per ml. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. For immunoprecipitation, the suggested use of this reagent is 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: When tested for ICC, W17231A displayed heterogeneous nuclear localization in HeLa cells. When tested for IP, W17231A detected minor, lower MW bands that were present in both input and IP fractions. These same bands were not observed in other WB experiments. We predict that these are minor degradation products due to slow lysis methods of extraction.
RRID: AB_2810681 (BioLegend Cat. No. 620601) AB_2810682 (BioLegend Cat. No. 620602)
Structure: TPX2 is a 747 amino acid protein with a predicted molecular weight of 86 kD.
Distribution: Nuclear, during mitosis strictly associated with spindle pole and mitotic spindle. In G2/S cell cycle, diffusely distributed throughout nucleus. Highly expressed in lung carcinoma cell lines, but not in normal lung tissues.
Function: Microtubule assembly
Biology Area: Cell Biology, Cell Cycle/DNA Replication, Cell Motility/Cytoskeleton/Structure, Cell Proliferation and Viability
Molecular Family: Microtubules
Antigen References: Fung T, et al. 2007. Mol Biol Cell. 2.042361111. Bibby R, et al. 2009. J Biol Chem. 284:33177. Warner S, et al. 2009. Clin Cancer Res. 5.152083333. Eot-Houllier G, et al. 2010. J Biol Chem. 285:29556. Tagal V, et al. 2017. Nat Commun. 8:14098.
Gene ID: 22974
UniProt: View information about TPX2 on UniProt.org
Clone: W17231A
Regulatory Status: RUO
Other Names: TPX2, Microtubule Nucleation Factor, Hepatocellular Carcinoma-Associated Antigen 519, Hepatocellular Carcinoma-Associated Antigen 90, P100, DIL2
Isotype: Rat IgG2a, λ