Biolegend, 621051, Purified anti-LEF1 Antibody, 25μg

Lymphoid Enhancing Factor 1 (LEF1) is a transcription factor belonging to the TCF/LEF family. LEF1 was first identified as a protein binding to TCR-α enhancer and upregulates expression of T-cell antigen receptor α chain. LEF1 is involved in Wnt signaling pathways as a ubiquitous regulator. During Wnt signaling, translocation of β-catenin from the cytosol to the nucleus results in elevation of transcriptional activity of LEF1, which subsequently regulates multiple downstream gene expression. LEF1 is implicated as a transcriptional regulator in the transformation of cancer cells in various diseases such as colonic adenocarcinoma. Due to its role in Wnt/β-catenin signaling pathway, LEF1 has been indicated as a facilitator of EMT and potential biomarker for various cancers.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant human LEF1 protein corresponding to amino acid residues 116-283
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: LEF-1 is known to have 7 isoforms. WB (Western blotting) or IHC-P (Immunohistochemistry FFPE) is not suggested for this product. For WB, IP (immunoprecipitation) or ICC (immunocytochemistry) applications, please refer to clones 15H5A18 (Cat. No. 653101/653102) or W17155A (Cat. No. 616001/616002). For ICFC (intracellular flowcytometry), True-Phos™ Perm Buffer (Cat. No. 425401) is suggested for permeabilization.
RRID: AB_3083406 (BioLegend Cat. No. 621051) AB_3083406 (BioLegend Cat. No. 621052)
Structure: LEF1 is a 399 amino acid protein with a predicted molecular weight of 44 kD. Seven total isoforms have been reported, ranging in size from 23 to 44 kD.
Distribution: Lymphoid tissue/Nuclear localization
Function: Canonical Wnt signaling
Interaction: Interacts with ALX4, AML-1, beta-catenin (CTNNB1), EP300, MITF, PIAS4, SMAD2, and SMAD3
Ligand/Receptor: Angiogenesis, Cell Biology, Immunology, Signal Transduction, Transcription Factors
Biology Area: Angiogenesis, Cell Biology, Immunology, Signal Transduction, Transcription Factors
Molecular Family: Nuclear Markers, TCRs
Antigen References: Mallory MJ, et al. 2011. Mol. Cell. Biol. 31:2184-95. Waterman ML, et al. 1991. Genes Dev. 5:656-69. Hovanes K, et al. 2001. Nat Genet. 28:53-7.. Bruhn L, et al. 1997. Genes Dev. 11:640-53. Nguyen A, et al. 2005. Int J Oncol. 27:949-56. Petropoulos K, et al. 2008. J Exp Med. 205:515-22.
Gene ID: 51176
UniProt: View information about LEF1 on UniProt.org
Clone: W17021C
Regulatory Status: RUO
Other Names: Lymphoid enhancer-binding factor 1, T cell-specific transcription factor 1-alpha (TCF1-alpha), TCF1α, TCF10, TCF7L3
Isotype: Rat IgG2a, λ