Biolegend, 621354, Alexa Fluor® 488 anti-Giantin Antibody, 200microl

Giantin is a membrane-inserted component of the cis and medial Golgi with a large rod-like cytoplasmic domain. This antibody serves as an excellent marker for the Golgi complex in mammalian cells.
200microl
Verified Reactivity: Human
Antibody Type: Polyclonal
Host Species: Rabbit
Immunogen: Recombinant fragment of human giantin N-terminus
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a dilution of 1:200 - 1:800 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Additional Product Notes: For Immunocytochemistry (ICC), the recommended fixation/permeabilization method is 4% paraformaldehyde/0.5% Triton X-100, or paraformaldehyde followed by ice-cold methanol, or ice-cold methanol fixed only.
RRID: AB_3699063 (BioLegend Cat. No. 621353)
Structure: Homodimer
Distribution: Widely express in tissues/localized to Golgi apparatus membrane
Function: Participate in forming intercisternal cross-bridges of the Golgi complex
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family: Golgi Markers
Antigen References: Day PM, et al. 2013. J. Virol. 87:3862-70. Linstedt AD and Hauri HP. 1993. Mol. Biol. Cell. 4:679-93.
Gene ID: 2804
UniProt: View information about Giantin on UniProt.org
Clone: Poly23A01
Regulatory Status: RUO
Other Names: Golgin subfamily B member 1, macrogolgin, golgi integral membrane protein 1, 372 kD Golgi complex-associated protein, golgin B1, golgi integral membrane protein, golgi autoantigen, golgin subfamily b, macrogolgin (with transmembrane signal)
Isotype: Rabbit Polyclonal IgG