Biolegend, 621632, KIRAVIA Blue 520™ anti-human CD279 (PD-1) Antibody, 100tests

Programmed cell death protein 1 (PD-1), also known as CD279, is a 55 kD member of the immunoglobulin superfamily. CD279 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region and plays a key role in peripheral tolerance and autoimmune disease. CD279 is expressed predominantly on activated T cells, B cells, and myeloid cells. PD-L1 (B7-H1, CD274) and PD-L2 (B7-DC, CD273) are ligands of CD279 (PD-1) and are members of the B7 gene family. Evidence suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. Interaction of CD279 ligands results in inhibition of T cell proliferation and cytokine secretion.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human CD279 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with KIRAVIA Blue 520™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * KIRAVIA Blue 520™ has an excitation maximum of 495 nm, and a maximum emission of 520 nm. KIRAVIA Blue™ 520 is a trademark of Sony. This product is subject to proprietary rights of Sony and is made and sold under license from Sony Corporation. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.Sony and the Sony logo are registered trademarks of Sony Corporation.
Excitation Laser: Blue Laser (488 nm)
Application Notes: A17188B antibody can block the binding of NAT105 and EH12.2H7 antibodies to the target.
RRID: AB_3097392 (BioLegend Cat. No. 621631) AB_3097392 (BioLegend Cat. No. 621632)
Structure: 55 kD type I transmembrane protein
Distribution: Transiently expressed on CD4- and CD8- thymocytes, upregulated in thymocytes and splenic T and B lymphocytes, and is expressed on activated myeloid cells.
Function: Signaling and co-stimulation (co-inhibition)
Interaction: SHP-1 and SHP-2
Ligand/Receptor: PD-L1 (CD274) and PD-L2 (CD273)
Cell Type: Lymphocytes, T cells
Biology Area: Cell Biology, Immuno-Oncology, Inhibitory Molecules
Molecular Family: CD Molecules
Antigen References: Ishida Y, et al. 1992. EMBO J. 11:3887 Francisco LM, et al. 2010. Immunol Rev. 236:219
Gene ID: 5133
UniProt: View information about CD279 on UniProt.org
Clone: A17188B
Regulatory Status: RUO
Other Names: PD1, PDCD1
Isotype: Mouse IgG2b, κ