Biolegend, 621701, Purified anti-STIM1 Antibody, 25μg

Stromal Interaction Molecule 1, or STIM1 is a single pass transmembrane, resident ER protein that facilitates extracellular calcium uptake following the depletion of intracellular stores. STIM1 directly senses loss of ER-lumenal calcium via an EF hand domain; elevated ER calcium levels stimulate redistribution of STIM1 to close proximity to the plasma mebrane, where it activates ORAI1, a pore component of the store-operated calcium entry system.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant human STIM1 protein corresponding to amino acid residues 436-685
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedICC, IP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. For immunoprecipitation, the suggested use of this reagent is 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: When testing W18094A testing for ICC, we observed poor staining of PFA-fixed cells that were permeabilized with methanol.When testing W18094A for WB, a minor non-specific band of ~50 kD was observed by WB at higher antibody concentrations.
RRID: AB_2814470 (BioLegend Cat. No. 621701) AB_2814471 (BioLegend Cat. No. 621702)
Structure: STIM1 is a 685 amino acid protein with a predicted molecular weight of 77 kD.
Distribution: ER Membrane/Ubiquitously expressed
Function: Calcium sensing and uptake
Biology Area: Cell Biology
Molecular Family: Innate Immune Signaling, Ion Channels
Antigen References: Roos J, et al. 2005. J. Cell Biol. 169:435. Peinelt C, et al. 2006. Nat Cell Biol. 8:771. Baba Y, et al. 2006. Proc Natl Acad Sci USA. 103:16704.
Gene ID: 6786
UniProt: View information about STIM1 on UniProt.org
Clone: W18094A
Regulatory Status: RUO
Other Names: Stromal Interaction Molecule 1, GOK, IMD10, TAM1, STRMK
Isotype: Rat IgG2b, λ