Biolegend, 621952, Purified anti-PPP1CA Antibody, 100μg

Protein phosphatase 1 catalytic subunit alpha (PPP1CA) is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine-specific protein phosphatase involved in the regulation of various cellular processes, such as cell division, glycogen metabolism, muscle contraction, protein synthesis, regulation of membrane receptors and channels, and HIV-1 virus transcription. PP1 has been shown to dephosphorylate RB and CDC25 during mitosis. Cell cycle-dependent phosphorylation at Thr320 of PPP1CA by CDC2 kinase inhibits PPP1CA activity. The studies in human and mice indicate that PP1 is an important regulator of cardiac function, and also show that PP1 suppresses learning and memory in mice.
100μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: A synthetic peptide of human PPP1CA
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The clone cross-reacts with mouse and rat in western blotting (WB). For immunocytochemistry (ICC), we recommend to use 4% PFA fixation followed by permeabilization with 0.5% Triton X-100 or ice cold-methanol. This product is not recommended for use in immunohistochemistry (IHC).
RRID: AB_3097497 (BioLegend Cat. No. 621951) AB_3097497 (BioLegend Cat. No. 621952)
Structure: PPP1CA protein is a 330 amino acid protein with a predicted molecular weight of 38 kD
Distribution: Upregulated in synovial fluid mononuclear cells and peripheral blood mononuclear cells from patients with rheumatoid arthritis
Interaction: FOXP3, CENPA, ATG16L1, TNS1, SAXO4, PPP1R21, PPP1R26, PPP1R27, PPP1R35, PPP1R36, PPP1R37, SH3RF2, ELFN1 and ELFN2
Cell Type: Neurons, Tregs
Biology Area: Cell Biology, Cell Cycle/DNA Replication, Cell Proliferation and Viability, Neuroscience, Protein Synthesis
Molecular Family: Protein Kinases/Phosphatase
Antigen References: Rubin E, et al.1998. Front Biosci. 3:D1209-19. Durfee T, et al. 1993. Genes Dev. 7:555-69. Izumi T, et al. 1992. Mol Biol Cell. 3:927-39. Kwon YG, et al. 1997. PNAS. 94:2168-73. Carr AN, et al. 2002. Mol Cell Biol. 22:4124-35. Genoux D, et al. 2002. Nature. 418:970-5.
Gene ID: 5499
UniProt: View information about PPP1CA on UniProt.org
Clone: W22109B
Regulatory Status: RUO
Other Names: PP1A, PP-1A, PPP1A, PP1alpha, PP1α
Isotype: Rat IgG2a, κ