Biolegend, 622205, PE anti-mouse TIGIT (Vstm3) Antibody, 25μg

TIGIT (T cell immunoreceptor with Ig and ITIM domains precursor) contains an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif. TIGIT is an inhibitory receptor expressed on the surface of natural killer (NK) cells. TIGIT recognizes nectin and nectin-like adhesion molecules and thus plays a critical role in the innate immune response to malignant transformation. TIGIT in conjunction with CD226 forms a pathway that has prominent similarities to the costimulatory CD28/CTLA-4 pathway. CD226 and TIGIT bind the same set of ligands (CD155 and CD112) and CD226 is a positive regulator of T cell responses, while TIGIT inhibits them. Knockdown of TIGIT expression in human CD4 + T cells results in an increase of both T-bet and IFN-γ mRNA and protein expression. These effects are overcome by blocking CD226 signaling indicating that TIGIT exerts immunosuppressive effects by competing with CD226 for the same ligand, CD155. The binding of CD155 to TIGIT on human dendritic cells enhances the production of interleukin 10 and diminishes the production of interleukin 12p40. TIGIT shows an inhibitory effect on cell-signaling pathway in murine models of experimental autoimmune encephalomyelitis (EAE). Loss of TIGIT expression in mice results in hyperproliferative T cell responses and increased susceptibility to EAE.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse TIGIT Recombinant Protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_2832837 (BioLegend Cat. No. 622205) AB_2832838 (BioLegend Cat. No. 622206)
Structure: Homodimer
Distribution: Human follicular B helper T cells (TFH), regulatory, memory and activated T cells, NK cells.
Function: Immunosuppressive effects by binding CD155 and modulating cytokine production by DC. Inhibits NK cell cytotoxicity. Inhibits T cell functions by competing with CD226. Tregs TIGIT+ cells suppress proinflammatory Th1 and Th17 cells, but not Th2 cell responses.
Interaction: Dendritic cells, monocytes
Ligand/Receptor: Poliovirus receptor (PVR/CD155) and nectin-2 (PRR-2/CD112)
Cell Type: NK cells, T cells
Biology Area: Cell Adhesion, Cell Biology, Immunology, Signal Transduction
Molecular Family: Adhesion Molecules, Immune Checkpoint Receptors, Soluble Receptors
Antigen References: Yu X, et al. 2009. Nat. Immunol. 10:48. Stanietsky N, et al. 2009. Proc. Natl. Acad. Sci. U S A. 106:17858. Joller N, et al. 2011. J. Immunol. 186:1338. Tahara-Hanaoka S, et al. 2004. Int. Immunol. 16:533. Lozano E, et al. 2012. J. Immunol. 188:3869. Joller N, et al. 2014. Immunity. 40:569. Deuss FA, et al. 2017. J. Biol. Chem. 292:11413.
Gene ID: 100043314
UniProt: View information about TIGIT on UniProt.org
Clone: A17200C
Regulatory Status: RUO
Other Names: T-Cell Immunoreceptor with Ig and ITIM domains, T cell Ig, Immunoglobulin Domain-Containing Protein 9, V-Set, Transmembrane Domain-Containing Protein 3, WUCAM
Isotype: Rat IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.