Biolegend, 622356, PE anti-human TSLPR Antibody, 100tests

TSLP-R, also known as the thymic stromal lymphopoietin protein receptor, cytokine receptor-like 2, CRL2, and IL-XR, is a type I membrane receptor that forms a functional heterodimeric complex with IL-7R to bind with TSLP. TSLP-R contains a WSXWS motif required for proper protein folding and a box 1 motif important for association with the JAKs. TSLP-R has a predicted molecular weight of approximately 40 kD, and has two isoforms reported that are produced by alternative splicing. The TSLP-R is expressed preferentially in myeloid cells, dendritic cells, and activated monocytes. It is weakly expressed on T cells. Expression has also been reported in heart, skeletal, muscle, and kidney tissues. TSLP binding to the heterodimeric functional receptor (TSLP-R and IL-7R) activates JAK2, STAT3, and STAT5 to stimulate cell proliferation. Ligand receptor interactions have been implicated in the development of the hematopoietic system, dendritic cell maturation, and the maintenance and polarization of human Th2 memory T cells in an allergic response.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant human TSLPR
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_3699064 (BioLegend Cat. No. 622355) AB_3699064 (BioLegend Cat. No. 622356)
Structure: Type I membrane receptor
Distribution: Expressed preferentially in myeloid cells including dendritic cells and activated monocytes, weakly expressed in T cells; also expressed in heart, skeletal, muscle, and kidney cells.
Function: TSLP binding to functional receptor activates JAK2, STAT3, and STAT5 to stimulate cell proliferation. It has also been implicated in the development of the hematopoietic system, dendritic cell maturation, and the maintenance and polarization of human Th2 memory T cells
Interaction: Forms functional heterodimeric complex with IL-7R to bind TSLP
Ligand/Receptor: TSLP/TSLPR
Cell Type: Dendritic cells, Monocytes, T cells, Tregs
Biology Area: Immunology
Molecular Family: Cytokine/Chemokine Receptors
Antigen References: Reche PA, et al. 2001. J Immunol. 167:336-43. Tonozuka Y, et al. 2001. Cytogenet. Cell. Genet. 93:23-5. Zhang W, et al. 2001. Biochem Biophys Res Commun. 281:878-83. Wang YH, et al. 2006. Immunity, 24:-838.
Gene ID: 64109
UniProt: View information about TSLPR on UniProt.org
Clone: W20296I
Regulatory Status: RUO
Other Names: Thymic stromal lymphopoietin protein receptor, cytokine receptor-like 2, CRL2, CRLF2, IL-XR, TSLP-R
Isotype: Rat IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.